García-Alvarado Francisco Javier, González-Martínez Marisela Del R, Jaramillo-Rodríguez Yolanda, Delgado-Aguirre Héctor Alberto
Facultad de Ciencias de la Salud, Universidad Juarez del Estado de Durango, Gomez Palacio, Mexico.
Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Coahuila, Torreón, México.
Biores Open Access. 2020 Feb 12;9(1):7-12. doi: 10.1089/biores.2019.0003. eCollection 2020.
Osteoarthritis (OA) of the knee causes disability, pain, and progressive destruction of cartilage in adult women. The objective of the study was to evaluate the concentrations of the urinary biomarker C-terminal telopeptide of type II collagen (CTX-II) and pain by radiographic grade in women with knee OA in northeastern Mexico: Cross-sectional study of 155 women with knee OA. Concentrations of biochemical parameters were evaluated and urine samples were collected to measure biomarker levels (uCTX-II) ng/mmol by competitive enzyme-linked immunoabsorbent assay (ELISA) technique and the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) scale was used for pain classification; median age of 49 years and 29.1 kg/m of body mass index (BMI). uCTX-II biomarker levels were grade 2 (210.7 ng/mmol), grade 3 (314.8 ng/mmol), and grade 4 (478.8 ng/mmol) relative to Kellgren and Lawrence, uCTX-II levels were compared with WOMAC scale and presented significant statistical difference ( = 0.0001). An association of the biomarker CTX-II and an increase in BMI was found in female patients with knee OA (odds ratio = 1.01; 95% confidence interval 1.001-1.005; = 0.047).This study demonstrates an increase in the levels of the biomarker uCTX-II, the degree of pain, and radiographic grade in women with knee OA in northeastern Mexico.
膝关节骨关节炎（OA）会导致成年女性残疾、疼痛以及软骨的渐进性破坏。本研究的目的是评估墨西哥东北部膝骨关节炎女性患者中尿生物标志物II型胶原C端肽（CTX-II）的浓度以及根据放射学分级评估疼痛情况：对155名膝骨关节炎女性患者进行横断面研究。评估生化参数浓度并收集尿液样本，采用竞争性酶联免疫吸附测定（ELISA）技术测量生物标志物水平（尿CTX-II），单位为ng/mmol，采用西安大略和麦克马斯特大学骨关节炎指数（WOMAC）量表进行疼痛分级；中位年龄为49岁，体重指数（BMI）为29.1kg/m²。相对于凯尔格伦和劳伦斯分级，尿CTX-II生物标志物水平在2级为（210.7ng/mmol）、3级为（314.8ng/mmol）、4级为（478.8ng/mmol），将尿CTX-II水平与WOMAC量表进行比较，呈现出显著的统计学差异（P = 0.0001）。在膝骨关节炎女性患者中发现生物标志物CTX-II与BMI增加之间存在关联（比值比 = 1.01；95%置信区间1.001 - 1.005；P = 0.047）。本研究表明，墨西哥东北部膝骨关节炎女性患者的生物标志物尿CTX-II水平、疼痛程度和放射学分级均有所增加。
