Toxicology Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Department of Toxicology, School of Pharmacy, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Environ Sci Pollut Res Int. 2020 May;27(13):15093-15102. doi: 10.1007/s11356-020-08048-0. Epub 2020 Feb 17.
Research has shown a relationship between the exposures to a chemical agent called bisphenol-A (BPA), which is extensively used in the production of polycarbonate plastics and the incidence of cardiovascular diseases. This association is most likely caused by the BPA's ability to disrupt multiple cardiac mechanisms, including mitochondrial functions. Therefore, this study aimed to explore the ability of quercetin (QUER) to limit the cardiotoxic effect of BPA in the rat's cardiac mitochondria. The experiment was carried out on 32 male Wistar rats, which were randomly assigned to four groups. The negative control group received olive oil; the positive control group received olive oil plus BPA (250 mg/kg); the third group received olive oil, BPA, and QUER (75 mg/kg); and the fourth group received olive oil and QUER, all orally for 14 days. The rats were slaughtered 24 h after the last treatment. The measured parameters included creatine kinase-MB (CK-MB) and lactate dehydrogenase (LDH) as the biomarkers of cardiotoxicity, triglyceride (TG), total cholesterol (TC), and low-density and high-density lipoprotein cholesterol (LDL-C and HDL-C) as the measures of dyslipidemia, glutathione (GSH) content, catalase activity (CAT), reactive oxygen species (ROS), lipid peroxidation (LPO), and the level of damage to the mitochondrial membranes as the indicators of the impact of QUER on the BPA cardiotoxic effect. Finally, the rats treated with QUER showed better results in terms of serum CK-MB, serum LDH, serum lipid profile, GSH level, CAT activity, mitochondrial membrane potential (ΔΨm), LPO, and ROS. According to the results, QUER could be used as a protective agent against BPA-induced mitochondrial toxicity.
研究表明，广泛用于生产聚碳酸酯塑料的化学物质双酚 A（BPA）的暴露与心血管疾病的发病率之间存在关联。这种关联很可能是由于 BPA 能够破坏多种心脏机制，包括线粒体功能。因此，本研究旨在探讨槲皮素（QUER）限制 BPA 对大鼠心脏线粒体的心脏毒性作用的能力。该实验在 32 只雄性 Wistar 大鼠上进行，将其随机分为四组。阴性对照组给予橄榄油；阳性对照组给予橄榄油加 BPA（250mg/kg）；第三组给予橄榄油、BPA 和 QUER（75mg/kg）；第四组给予橄榄油和 QUER，均连续口服 14 天。最后一次处理后 24 小时处死大鼠。测量的参数包括肌酸激酶同工酶-MB（CK-MB）和乳酸脱氢酶（LDH）作为心脏毒性的生物标志物，甘油三酯（TG）、总胆固醇（TC）和低密度和高密度脂蛋白胆固醇（LDL-C 和 HDL-C）作为血脂异常的指标，谷胱甘肽（GSH）含量、过氧化氢酶活性（CAT）、活性氧（ROS）、脂质过氧化（LPO）和线粒体膜损伤程度作为 QUER 对 BPA 心脏毒性作用影响的指标。最后，给予 QUER 的大鼠在血清 CK-MB、血清 LDH、血清脂质谱、GSH 水平、CAT 活性、线粒体膜电位（ΔΨm）、LPO 和 ROS 方面表现出更好的结果。根据结果，QUER 可用作对抗 BPA 诱导的线粒体毒性的保护剂。
