Yao Y Y, Xiong C R, Dong X, Li W, Zhang J F, Yang K
National Health Commission Key Laboratory of Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology, Jiangsu Institute of Parasitic Diseases, Wuxi 214064, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2019 Nov 22;31(6):652-654. doi: 10.16250/j.32.1374.2019205.
To evaluate the value of a dynamic automatic identification system in routine miracidium hatching test with nylon gauzes.
Different quantities of fresh eggs were added to bovine fecal samples and divided into the low-infection group, medium-infection group and high-infection group, while the bovine feces without eggs served as negative controls. The detection efficiency and accuracy were compared between the identification system and manual detection in different groups.
The identification system can automatically identify miracidium. The detection rate and efficiency of miracidium in bovine fecal samples were both higher by using the identification system than by manual detection. Notably in the low-infection group, the identification system had a significantly higher rate of detection of miracidium than manual detection (χ = 10.769, = 0.002). The identification system completed the detection of bovine fecal samples in the field within 1 min.
The dynamic automatic identification system may effectively improve the detection efficiency and accuracy of routine miracidium hatching test with nylon gauzes, and it may replace manual detection to be used in the field schisotsomiasis examinations and related researches.
评估动态自动识别系统在尼龙绢集卵孵化法常规毛蚴孵化试验中的应用价值。
向牛粪样本中加入不同数量的新鲜虫卵,分为低感染组、中感染组和高感染组,未加虫卵的牛粪作为阴性对照。比较不同组中识别系统与手工检测的检测效率和准确性。
该识别系统能够自动识别毛蚴。使用识别系统检测牛粪样本中毛蚴的检出率和效率均高于手工检测。值得注意的是,在低感染组中,识别系统检测毛蚴的阳性率显著高于手工检测(χ² = 10.769,P = 0.002)。该识别系统可在1分钟内完成现场牛粪样本的检测。
动态自动识别系统可有效提高尼龙绢集卵孵化法常规毛蚴孵化试验的检测效率和准确性,有望取代手工检测应用于现场血吸虫病检测及相关研究。