Jian-Rong Dai, Xue-Hui Shen, Guo-Li Qu, Yi-An Wang, Yun-Tian Xing, Wei Wang, You-Sheng Liang
Jiangsu Institute of Parasitic Diseases, Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention, Jiangsu Provincial Key Laboratory on Parasites and Vector Control Technology, Wuxi 214064, China.
Dantu District Center for Disease Control and Prevention, Zhenjiang City, Jiangsu Province, China.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi. 2016 Oct 20;28(5):485-489. doi: 10.16250/j.32.1374.2016174.
To develop a simple, feasible goat feces collector to improve the collection accuracy and integrity of goat fecal samples without pollution, and to modify the miracidium hatching test with a plastic tube to achieve simple, standard and comparative procedures, so as to provide technical support for pathogenic diagnosis and scientific research of goat schistosomiasis japonica.
According to the body features of goat in marshland regions, a goat fecal collector, which was made of coarse fabric cottons, was devised, which was able to be fixed onto the goat buttocks and avoid urine pollution. Prior to miracidium hatching test, the goat fecal samples were pieced by using a mechanical method instead of the conventional artificial piecing method, and the effect of mechanical piecing treatment on miracidium hatching was evaluated. A filter membrane was added between the tube and rubbery ring to block the floater in fecal residues into the tube. The effects on miracidium hatching by using thin fat-free cotton, thick fat-free cotton, nylon gauze at 100 pores/25.4 mm and 150 pores/25.4 mm were compared.
The goat feces collector was composed of foreleg fixing garment, hindleg fixing garment and stool bag. The functions of the fixing garment were as a fixed collector to allow non-shift and tolerance of weight during goat activity, while the major function of stool bag was in storage of stool. The goat activity did not affect by the use of collector, and all fecal samples were excreted to the bag. This collector was easy to perform and could avoid urine pollution, which was reusable after cleaning. Prior to miracidium hatching, the goat fecal samples, together with water, were pieced at 18000 to 23000 r/min for successive three times in a cooking machine, of 10 s each time at an interval of 5 s. Mechanical piecing had no clear-cut effect on miracidium hatching of eggs in fecal samples. A total of 541, 620, 344 and 211 miracidia were detected by using the miracidium hatching test with nylon gauze at 100 pores/25.4 mm and 150 pores/25.4 mm, thin fat-free cotton and thick fat-free cotton respectively, indicating a better detection efficacy by using nylon gauze at 100 pores/25.4 mm and 150 pores/25.4 mm.
The goat fecal collector is a easy-to-perform, accurate, unpolluted and reusable device to collect goat feces, which is suitable for pathogenic diagnosis of goat schistosomiasis. Mechanical piecing and use of nylon gauze at 150 pores/25.4 mm allow a simple, accurate and stable technique for parasitological diagnosis of schistosomiasis japonica, which provides a reliable tool for schistosomiasis control and research.
研制一种简单、可行的山羊粪便收集器，提高山羊粪便样本收集的准确性和完整性，避免污染，并对塑料管毛蚴孵化试验进行改进，实现操作简单、规范且具有可比性的流程，为山羊日本血吸虫病的病原诊断和科研提供技术支持。
根据沼泽地区山羊的身体特征，设计一种由粗棉布制成的山羊粪便收集器，该收集器能够固定在山羊臀部，避免尿液污染。在毛蚴孵化试验前，采用机械方法代替传统的人工破碎方法对山羊粪便样本进行破碎，并评估机械破碎处理对毛蚴孵化的影响。在管与橡胶圈之间添加滤网，阻止粪便残渣中的漂浮物进入管内。比较使用薄脱脂棉、厚脱脂棉、100目/25.4mm和150目/25.4mm尼龙纱布对毛蚴孵化的影响。
山羊粪便收集器由前腿固定衣、后腿固定衣和粪便袋组成。固定衣的作用是作为固定收集器，使山羊活动时不发生移位并能承受重量，而粪便袋的主要功能是储存粪便。使用收集器不影响山羊活动，所有粪便样本均排入袋中。该收集器操作简便，可避免尿液污染，清洗后可重复使用。在毛蚴孵化前，将山羊粪便样本与水在料理机中以18000至23000转/分钟连续破碎3次，每次10秒，间隔5秒。机械破碎对粪便样本中虫卵的毛蚴孵化无明显影响。使用100目/25.4mm和150目/25.4mm尼龙纱布、薄脱脂棉和厚脱脂棉进行毛蚴孵化试验分别检测到541、620、344和
