Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan.
Department of Biotechnology and Genetic Engineering, Kohat University of Science and Technology, Kohat, Pakistan.
Int J Phytoremediation. 2020;22(6):653-661. doi: 10.1080/15226514.2019.1707160. Epub 2020 Feb 16.
Novel technologies are required for rapid reclamation of saline soils. The halotrophic and chitinolytic bacterial strains were used for phytoremediation of saline soils using spinach plants ( L.). The previously isolated chitinolytic bacteria showed high antifungal potential against and The halotolerant bacterial strains were previously isolated showing a salt tolerance of up to 20% in culture media. Specially designed microcosms were used here to investigate the reclamation of saline soil by bacteria. The soil salinity was reduced by both types of bacteria (from 6.5 to 2 dS/m). A decline in Na contents from 22-24 to 9-12 meq/L and in sodium adsorption ratio from 10-11 to 7-8 was also observed in saline soils. The Ca/Mg contents increased from 24 to 30-33 meq/L. The bioassays were performed to evaluate the effect of the bacteria on the phytoremediation. The shoot, root weights (both fresh (1.927 g, 0.244 g) and dry (0.387 g, 0.104 g)) increased by bacterial inoculation as compared to control in saline soils. The Na/K ratio decreased in plant tissues. Here we report the increased efficacy of phytoremediation by combined inoculation of chitinolytic and halotolerant bacterial strains in soil which has never been reported before.
需要新型技术来快速改良盐渍土。利用菠菜（ L.）植物，利用盐生和几丁质分解细菌菌株进行盐渍土的植物修复。先前分离的几丁质分解细菌对 和 表现出高抗真菌潜力。先前分离的耐盐细菌菌株在培养基中表现出高达 20%的耐盐性。专门设计的微宇宙用于研究细菌对盐渍土的改良。两种类型的细菌（从 6.5 到 2 dS/m）降低了土壤盐分。在盐渍土中，Na 含量从 22-24 下降到 9-12 meq/L，钠吸附比从 10-11 下降到 7-8。Ca/Mg 含量从 24 增加到 30-33 meq/L。进行生物测定以评估细菌对植物修复的影响。与对照相比，细菌接种后，盐渍土中的植物地上部和根重（鲜重（1.927 g，0.244 g）和干重（0.387 g，0.104 g））增加。植物组织中的 Na/K 比值降低。在这里，我们报告了在土壤中联合接种几丁质分解和耐盐细菌菌株可提高植物修复的功效，这是以前从未报道过的。
