Chadwick Jeffrey W, Glogauer Michael
Department of Dental Oncology and Maxillofacial Prosthetics, Princess Margaret Cancer Centre, University Health Network; Faculty of Dentistry, University of Toronto.
Department of Dental Oncology and Maxillofacial Prosthetics, Princess Margaret Cancer Centre, University Health Network; Faculty of Dentistry, University of Toronto;
J Vis Exp. 2020 Jan 21(155). doi: 10.3791/59667.
The main advantages of studying the pathophysiology of periodontal disease utilizing murine models are the reduced cost of animals, array of genetically modified strains, the vast number of analyses that can be performed on harvested soft and hard tissues. However, many of these systems are subject to procedural criticisms. As an alternative, the ligature-induced model of periodontal disease, driven by the localized development and retention of a dysbiotic oral microbiome, can be employed, which is rapidly induced and relatively reliable. Unfortunately, the variants of ligature-induced murine periodontitis protocol are isolated to focal regions of the periodontium and subject to premature avulsion of the installed ligature. This minimizes the amount of tissue available for subsequent analyses and increases the number of animals required for study. This protocol describes the precise manipulations required to place extended molar ligatures with improved retention and usage of a novel rinse technique to recover oral neutrophils in mice with an alternative approach that mitigates the aforementioned technical challenges.
利用小鼠模型研究牙周疾病病理生理学的主要优点包括动物成本降低、有一系列基因改造菌株以及可对采集的软硬组织进行大量分析。然而，许多此类系统受到操作方面的批评。作为一种替代方法，可以采用由失调口腔微生物群的局部发育和存留驱动的结扎诱导型牙周疾病模型，该模型诱导迅速且相对可靠。不幸的是，结扎诱导型小鼠牙周炎方案的变体局限于牙周的局部区域，并且安装的结扎线容易过早脱落。这减少了可用于后续分析的组织量，并增加了研究所需的动物数量。本方案描述了放置延长磨牙结扎线所需的精确操作，以提高结扎线的存留率，并采用一种新颖的冲洗技术，通过另一种方法在小鼠中回收口腔中性粒细胞，从而减轻上述技术挑战。
