Department of Stomatology, The Fourth Affiliated Hospital, Harbin Medical University, Harbin, 150001, China.
Faculty of Dentistry, Department of Oral Biological and Medical Sciences, University of British Columbia, Vancouver, BC, V6T 1Z3, Canada.
Lasers Med Sci. 2021 Apr;36(3):675-680. doi: 10.1007/s10103-020-03126-8. Epub 2020 Aug 17.
This study aimed to evaluate the effects of toluidine blue-mediated photodynamic therapy (TB-PDT) on the periodontitis-induced bone resorption in periodontitis in rats. Periodontal disease was induced by cotton ligature around the right second maxillary molar in 64 rats. After 4 weeks, the rats were randomly divided into four groups: sterile saline solution (control group); laser therapy (laser group); TB (100 μg/mL); TB plus laser (0.15 W/cm) irradiation every other day for 240 s (PDT group). All rats were euthanized at 15 days postoperatively. Eight gingival tissue samples were collected from each group. The expressions of receptor activator of nuclear factor kappa-Β ligand (RANKL) and osteoprotegerin (OPG) in gingival tissue samples were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The maxillae from the rest of the rats were taken for histological examination. In the PDT group, the analysis revealed less bone loss than in the control treatment (P < 0.05). No significant difference was found among the control group, TB group, and laser group (P > 0.05). Significantly higher and lower expressions of RANKL and OPG were revealed in the PDT group than that in control group, respectively (P < 0.01). When compared with the control group, the expression of RANKL was significantly reduced by 40.0% in periodontitis in rats treated with TB-PDT for 15 days (P < 0.01). The expression of OPG was increased in the PDT group with TB-PDT for 15 days, when compared with the control group (P < 0.05). TB-PDT treatment significantly reverses the abnormal expression of RANKL and OPG in periodontitis in rats.
本研究旨在评估甲苯胺蓝介导的光动力疗法（TB-PDT）对大鼠牙周炎诱导的骨吸收的影响。通过在 64 只大鼠右侧上颌第二磨牙周围放置棉线结扎来诱导牙周病。4 周后，大鼠随机分为四组：无菌生理盐水溶液（对照组）；激光治疗（激光组）；TB（100μg/mL）；TB 加激光（0.15W/cm），每隔一天照射 240s（PDT 组）。所有大鼠均在手术后 15 天处死。每组收集 8 个牙龈组织样本。通过实时定量聚合酶链反应（RT-qPCR）检测牙龈组织样本中核因子κB 受体激活配体（RANKL）和骨保护素（OPG）的表达。其余大鼠的上颌骨用于组织学检查。在 PDT 组中，分析显示与对照组相比骨丢失减少（P<0.05）。对照组、TB 组和激光组之间无显著差异（P>0.05）。PDT 组的 RANKL 和 OPG 表达明显高于对照组（P<0.01）。与对照组相比，TB-PDT 治疗 15 天的大鼠牙周炎中 RANKL 的表达显著降低了 40.0%（P<0.01）。与对照组相比，TB-PDT 治疗 15 天的大鼠牙周炎中 OPG 的表达增加（P<0.05）。TB-PDT 治疗可显著逆转大鼠牙周炎中 RANKL 和 OPG 的异常表达。
