甲硫氨酸酶链反应：一种基于酶的自催化扩增系统，用于检测硫醇。
The methionase chain reaction: an enzyme-based autocatalytic amplification system for the detection of thiols.
机构信息
Department of Chemical and Biomolecular Engineering, University of California, Berkeley, Berkeley, CA 94720, USA.
出版信息
Chem Commun (Camb). 2020 Mar 14;56(21):3175-3178. doi: 10.1039/c9cc09136j. Epub 2020 Feb 17.
Abstract
We present an autocatalytic system for the detection and amplification of thiols termed the Methionase Chain Reaction (MCR). MCR is based on the reversible modification of the thiol producing enzyme Methionine Gamma-Lyase (MGL). MCR was able to amplify the concentration of thiols by a factor of 560 and was able to visually detect thiols at concentrations as low as 50 nM.
摘要
我们提出了一种称为硫醇自催化检测和扩增的体系，即甲硫氨酸γ-裂合酶反应（MCR）。MCR 基于对产生硫醇的酶甲硫氨酸γ-裂合酶（MGL）的可逆修饰。MCR 能够将硫醇的浓度放大 560 倍，并能够在低至 50 nM 的浓度下可视化检测硫醇。
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