一种酶介导的扩增策略可直接在未经处理的临床样本中检测β-内酰胺酶活性，从而实现对β-内酰胺耐药性的表型检测。
An Enzyme-Mediated Amplification Strategy Enables Detection of β-Lactamase Activity Directly in Unprocessed Clinical Samples for Phenotypic Detection of β-Lactam Resistance.
机构信息
Department of Bioengineering, University of California, Berkeley, University Avenue, Berkeley, CA, 94720, USA.
Department of Public Health, University of California, Berkeley, University Avenue, Berkeley, CA, 94720, USA.
出版信息
Chembiochem. 2018 Oct 18;19(20):2173-2177. doi: 10.1002/cbic.201800443. Epub 2018 Sep 26.
Abstract
Biochemical assays that can identify β-lactamase activity directly from patient samples have the potential to significantly improve the treatment of bacterial infections. However, current β-lactamase probes do not have the sensitivity needed to measure β-lactam resistance directly from patient samples. Here, we report the development of an instrument-free signal amplification technology, DETECT, that connects the activity of two enzymes in series to effectively amplify the activity of β-lactamase 40 000-fold, compared to the standard β-lactamase probe nitrocefin.
摘要
能够直接从患者样本中识别β-内酰胺酶活性的生化分析方法有可能显著改善细菌感染的治疗效果。然而，目前的β-内酰胺酶探针没有从患者样本中直接测量β-内酰胺耐药性所需的灵敏度。在这里，我们报告了一种无仪器信号放大技术 DETECT 的开发，该技术将两种酶的活性串联起来，与标准的β-内酰胺酶探针硝噻吩相比，将β-内酰胺酶的活性有效放大了 40000 倍。
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