Laboratory of Bioprocess Technology, Department of Microbiology, Maharshi Dayanand University, Rohtak, Haryana, 124001, India.
Department of Biotechnology, Central University of Haryana, Jant-Pali, Mahendergarh, Haryana, 123031, India.
Bioprocess Biosyst Eng. 2020 Jun;43(6):1081-1091. doi: 10.1007/s00449-020-02306-8. Epub 2020 Feb 17.
An investigation was carried out using sugarcane bagasse as the agricultural residue to study the optimization of xylanase production by solid-state fermentation. Maximum xylanase production (20.35 U/g substrate) was achieved by Bacillus substilis subsp. subtilis JJBS250 using 'one variable at a time approach' at pH 7.0, 40 °C after 48 h. After statistical optimization by response surface methodology (RSM) there was 4.82-fold improvement in xylanase production (98.16 U/g substrate). Further optimization of untreated and sodium carbonate pretreated sugarcane bagasse enzymatic hydrolysis was carried out using both bacterial (Bacillus substilis subsp. subtilis JJBS250) and fungal (Myceliophthora thermophila BJTLRMDU3) xylanases that showed high amount of reducing sugar liberation from untreated sugarcane bagasse (124.24 mg/g substrate) as compared to pretreated (76.23 mg/g substrate) biomass. Furthermore, biophysical characterization of untreated and sodium carbonate pretreated sugarcane bagasse using Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD) and scanning electron microscopy (SEM), revealed the structural changes in the pretreated biomass.
采用甘蔗渣作为农业残余物进行了一项研究，旨在通过固态发酵优化木聚糖酶的生产。使用“单变量法”在 pH 值为 7.0、40°C 的条件下，经过 48 小时，芽孢杆菌亚种。subtilis JJBS250 实现了最大木聚糖酶产量（20.35 U/g 基质）。通过响应面法（RSM）进行统计学优化后，木聚糖酶产量提高了 4.82 倍（98.16 U/g 基质）。进一步使用细菌（芽孢杆菌亚种。subtilis JJBS250）和真菌（嗜热毁丝霉 BJTLRMDU3）木聚糖酶对未经处理和碳酸钠预处理的甘蔗渣进行了酶解优化，与预处理（76.23 mg/g 基质）生物质相比，未经处理的甘蔗渣释放出更多的还原糖（124.24 mg/g 基质）。此外，使用傅里叶变换红外光谱（FTIR）、X 射线衍射（XRD）和扫描电子显微镜（SEM）对未经处理和碳酸钠预处理的甘蔗渣进行了生物物理特性分析，揭示了预处理生物质的结构变化。
