Vinogradov A E, Ezhevskiĭ Iu M, Rozanov Iu M, Kazhdan I Ia, Tsveĭbakh A S
Tsitologiia. 1988 Aug;30(8):983-90.
Patterns of the cell cycle distribution in human peripheral blood lymphocytes, stimulated by PHA alone and PHA plus 12-o-tetradecanoylphorbol-13-acetate (TPA), were studied using DNA cytometry in different times after PHA stimulation. In the first period (nearly 3 days after PHA stimulation) TPA induces no significant differences in the characters under consideration, but in the later period, when the proliferation of the cultures stimulated by PHA alone is reducing, in other cultures stimulated by PHA plus TPA the percentage of cells in S-phase does not reduce, whereas the percentage of cells in G2-phase is rising, which may suggest that this phase is blocked. Concurrently the tetraploid cells are appearing. Accumulation of cells in G2-phase can be overcome by the application of chlorpromazine, which is known to inhibit the membrane-associated protein kinase C.
采用DNA细胞计量术研究了仅用PHA以及PHA加12 - O - 十四烷酰佛波醇 - 13 - 乙酸酯（TPA）刺激的人外周血淋巴细胞在PHA刺激后不同时间的细胞周期分布模式。在第一个时期（PHA刺激后近3天），TPA在所研究的特征方面未引起显著差异，但在后期，当仅用PHA刺激的培养物增殖减少时，在其他用PHA加TPA刺激的培养物中，S期细胞的百分比并未降低，而G2期细胞的百分比在上升，这可能表明该期被阻断。同时出现了四倍体细胞。应用氯丙嗪可克服G2期细胞的积累，已知氯丙嗪可抑制与膜相关的蛋白激酶C。
