Fuhrman Conti A M, Tori R, Ronchetti E, De Grada L, Pellicciari C, Manfredi Romanini M G
Dipartimento di Biologia e Genetica per le Scienze Mediche, University of Milano, Italy.
Basic Appl Histochem. 1990;34(4):269-79.
The effect of prolonged exposure to a hypertonic medium on human lymphocytes during mitogenic stimulation with phytohemagglutinin was investigated. The process of chromatin decondensation during the first 24 hrs stimulation (G0 to G1 transition) and the changes in kinetic parameters and the occurrence of chromosome aberrations from 48 hrs to 72 hrs of stimulation were studied. In HT medium, lymphocyte transition from G0 to G1 was slowed; there were fewer S-phase cells, after 48 hrs PHA stimulation, whereas after 72 hrs the resistant cells showed the same frequency of S-phase cells as the controls. The mitotic index was always smaller, and the frequency of G0/G1 cells larger. No significant increase in the frequencies of chromosome aberrations were found. These findings suggest that human peripheral lymphocytes can survive and grow in a hypertonic medium; chromosome damages, if not repaired, may be lethal, and only lymphocytes with normal karyotypes can survive for long times in the HT medium, although with modified kinetic characteristics.
研究了在植物血凝素促有丝分裂刺激过程中，长时间暴露于高渗培养基对人淋巴细胞的影响。研究了刺激前24小时（从G0期到G1期转变）染色质解聚的过程，以及刺激48小时至72小时期间动力学参数的变化和染色体畸变的发生情况。在HT培养基中，淋巴细胞从G0期到G1期的转变减慢；在PHA刺激48小时后，S期细胞较少，而在72小时后，抗性细胞的S期细胞频率与对照组相同。有丝分裂指数始终较小，G0/G1期细胞频率较大。未发现染色体畸变频率有显著增加。这些发现表明，人外周淋巴细胞可以在高渗培养基中存活和生长；染色体损伤若不修复可能是致命的，只有核型正常的淋巴细胞才能在HT培养基中长时间存活，尽管其动力学特征有所改变。
