Department of Hydrobiology, Faculty of Biology, University of Warsaw at Biological and Chemical Research Centre, Żwirki i Wigury 101, 02-089, Warsaw, Poland; Department of Biochemistry and Microbiology, Institute of Biology, Warsaw University of Life Sciences (SGGW), Nowoursynowska 159, 02-776, Warsaw, Poland.
Department of Hydrobiology, Faculty of Biology, University of Warsaw at Biological and Chemical Research Centre, Żwirki i Wigury 101, 02-089, Warsaw, Poland.
Environ Pollut. 2020 Jun;261:114135. doi: 10.1016/j.envpol.2020.114135. Epub 2020 Feb 10.
The effect of chronic exposure of freshwater cladoceran Daphnia magna to low, environmentally relevant concentrations i.e 4 μgLof ibuprofen (a nonsteroidal anti-inflammatory drug) in a laboratory experiment was studied. We observed the key life history traits of first and fifth generation individuals: age and size at first reproduction, number of first clutch eggs and individual growth rate. Moreover, chosen molecular/subcellular markers of experimental animals stress response such as triglyceride content, heat shock proteins (HSP) expression and DNA:RNA ratio were collected. Overall, chronic exposure to ibuprofen had no significant effect on the molecular markers nor on the life history parameters of the Daphnia. It did, however, caused lethal morphological deformities in embryos and juvenile daphnids. Depending on the clonal affiliation, exposure to a low dosage of ibuprofen over five generations resulted in the deformation of ∼3%-∼10% of the first clutch of offspring. Also, up to 90% of females carried at least one deformed embryo. This is the first time that research has revealed such an effect of ibuprofen on D. magna.
在实验室实验中，研究了淡水枝角类动物大型溞（Daphnia magna）在慢性暴露于低浓度（即 4 μgL）环境相关浓度的布洛芬（一种非甾体抗炎药）下的影响。我们观察了第一代和第五代个体的关键生活史特征：首次繁殖的年龄和大小、第一窝卵的数量和个体增长率。此外，还收集了实验动物应激反应的选择分子/亚细胞标志物，如甘油三酯含量、热休克蛋白（HSP）表达和 DNA:RNA 比。总体而言，慢性暴露于布洛芬对分子标志物和溞的生活史参数均没有显著影响。然而，它确实导致了胚胎和幼年溞类的致命形态畸形。根据克隆隶属关系，在五代内接触低剂量的布洛芬会导致第一窝后代中约 3%-10%的畸形。此外，高达 90%的雌性至少携带一个畸形胚胎。这是首次研究表明布洛芬对大型溞有这种影响。
