Institute of Infection & Global Health, University of Liverpool, Neston, Cheshire, UK
Institute of Infection & Global Health, University of Liverpool, Neston, Cheshire, UK.
Vet Rec. 2020 Apr 18;186(14):450. doi: 10.1136/vr.105628. Epub 2020 Feb 17.
Non-healing bovine foot lesions, including non-healing white line disease, non-healing sole ulcer and toe necrosis, are an increasingly important cause of chronic lameness that are poorly responsive to treatment. Recent studies have demonstrated a high-level association between these non-healing lesions and the phylogroups implicated in bovine digital dermatitis (BDD). However, a polymicrobial aetiology involving other gram-stain-negative anaerobes is suspected.
A PCR-based bacteriological survey of uncomplicated BDD lesions (n=10) and non-healing bovine foot lesions (n=10) targeting , and was performed.
DNA was detected in 80.0% of the non-healing lesion biopsies (p=<0.001) but was entirely absent from uncomplicated BDD lesion biopsies. When compared to the BDD lesions, was detected at a higher frequency in the non-healing lesions (33.3% vs 70.0%, respectively), whereas was detected at a lower frequency (55.5% vs 20.0%, respectively). Conversely, DNA was not detected in either lesion type.
The data from this pilot study suggest that and should be further investigated as potential aetiological agents of non-healing bovine foot lesions. A failure to detect syphilis treponemes in either lesion type is reassuring given the potential public health implications such an infection would present.
非愈合牛脚病变，包括非愈合白线病、非愈合鞋底溃疡和趾坏死，是一种日益重要的慢性跛行原因，对治疗反应不佳。最近的研究表明，这些非愈合病变与涉及牛传染性趾间皮炎（BDD）的系统发育群之间存在高度关联。然而，据怀疑，一种涉及其他革兰氏阴性厌氧菌的混合微生物病因。
针对 和 ，对非复杂性 BDD 病变（n=10）和非愈合牛脚病变（n=10）进行基于 PCR 的细菌学调查。
80.0%的非愈合病变活检中检测到 DNA（p=<0.001），但在非复杂性 BDD 病变活检中完全不存在。与 BDD 病变相比，非愈合病变中 检测到的频率更高（分别为 33.3%和 70.0%），而 检测到的频率更低（分别为 55.5%和 20.0%）。相反，两种病变类型均未检测到 DNA。
这项初步研究的数据表明， 和 应进一步研究作为非愈合牛脚病变的潜在病因。鉴于这种感染可能带来的公共卫生影响，在两种病变类型中均未检测到梅毒螺旋体是令人放心的。
