International Islamic University Islamabad, Islamabad, Pakistan.
Cell Tissue Bank. 2020 Jun;21(2):249-256. doi: 10.1007/s10561-020-09817-4. Epub 2020 Feb 17.
Radiation sterilization is an effective method of bone sterilization prior to bone graft transplantation. Gamma irradiation affects the biological and mechanical properties of bone; depending on the dose of radiation. The effect of gamma irradiation on bone mechanical properties is an unwanted phenomenon. However the mechanism of the effect of irradiation on bone mechanical properties is not properly understood. In this research paper the mechanism of the effect of gamma irradiation on bovine bone is investigated using scanning electron microscopy, energy-dispersive X-rays spectroscopy and Fourier transform infrared spectroscopy techniques. Gamma irradiation affects the mineral and fiber composition of bovine bone. The mineral content of bone especially calcium, magnesium and phosphorus decrease with increasing dose of gamma radiation. At Nano-level gamma irradiation alter amide I, amide II and amide III collagen contents. High dose gamma irradiation induces collagen cross-linking reaction in bone and degrades bone properties.
辐照灭菌是骨移植前对骨进行灭菌的有效方法。伽马辐照会影响骨骼的生物和机械性能；这取决于辐射剂量。伽马辐照对骨机械性能的影响是一种不期望的现象。然而，辐照对骨机械性能影响的机制尚未得到很好的理解。在本研究论文中，使用扫描电子显微镜、能谱仪和傅里叶变换红外光谱技术研究了伽马辐照对牛骨的影响机制。伽马辐照会影响牛骨的矿物质和纤维组成。骨中的矿物质含量，特别是钙、镁和磷，随着伽马辐射剂量的增加而减少。在纳米级水平上，伽马辐照会改变 I 型酰胺、II 型酰胺和 III 型酰胺胶原蛋白的含量。高剂量伽马辐照会诱导骨中的胶原交联反应，从而降低骨的性能。
