Department of Thoracic Surgery, China-Japan Union Hospital of Jilin University, Changchun, China.
Department of Orthopedics, China-Japan Union Hospital of Jilin University, Changchun, China.
Phytother Res. 2020 Jul;34(7):1629-1637. doi: 10.1002/ptr.6629. Epub 2020 Feb 17.
Curcumin (CUR) is a kind of polyphenolic compound and widely used in the treatment of diseases. However, the involvement of CUR in thymic carcinoma remains unknown. The object of our research is to clarify the role of CUR and related regulatory mechanism in thymic carcinoma cells. After treatment with CUR for 24 hr, cell viability, apoptosis, migration, and invasion of TC1889 cells were measured. Real-time polymerase chain reaction was executed to examine the expression of microRNA-27a (miR-27a) in thymic carcinoma tissues and TC1889 cells. After miR-27a mimic transfection, whether miR-27a is involved in CUR-modulated cell behaviors was measured. Finally, western blot was utilized to detect mTOR and Notch 1 pathways-linked proteins. CUR restrained cell viability and increased cell apoptosis of TC1889 cells. In addition, cell migration and invasion were restrained by CUR. Meanwhile, miR-27a expression was positively regulated in thymic carcinoma tissues and downregulated by CUR in TC1889 cells. Overexpressed miR-27a reversed the CUR-induced reduction of growth, migration, and invasion in TC1889 cells. Furthermore, CUR blocked mTOR and Notch 1 pathways via downregulating miR-27a. We demonstrated that CUR blocked mTOR and Notch 1 pathways via downregulating miR-27a, thereby suppressing cell growth, migration, and invasion of thymic carcinoma cells.
姜黄素（CUR）是一种多酚化合物，广泛用于治疗疾病。然而，CUR 参与胸腺癌的情况尚不清楚。我们的研究目的是阐明 CUR 及相关调节机制在胸腺癌细胞中的作用。用 CUR 处理 24 小时后，测量 TC1889 细胞的活力、凋亡、迁移和侵袭。实时聚合酶链反应检测胸腺癌组织和 TC1889 细胞中 microRNA-27a（miR-27a）的表达。转染 miR-27a 模拟物后，测量 miR-27a 是否参与 CUR 调节的细胞行为。最后，用 Western blot 检测 mTOR 和 Notch 1 通路相关蛋白。CUR 抑制 TC1889 细胞的活力并增加细胞凋亡。此外，CUR 抑制细胞迁移和侵袭。同时，miR-27a 在胸腺癌组织中呈正调控，在 TC1889 细胞中被 CUR 下调。过表达 miR-27a 逆转了 CUR 诱导的 TC1889 细胞生长、迁移和侵袭减少。此外，CUR 通过下调 miR-27a 阻断 mTOR 和 Notch 1 通路。我们证明 CUR 通过下调 miR-27a 阻断 mTOR 和 Notch 1 通路，从而抑制胸腺癌细胞的生长、迁移和侵袭。
