The College of Life Sciences , Northwest University , Xi'an, P.R. China Key Laboratory of Resource Biology and Biotechnology in Western China , Northwest University , Xi'an , P.R. China.
Pol J Microbiol. 2020;69(1):1-10. doi: 10.33073/pjm-2020-006.
Hydrogen formed during nitrogen fixation in legumes can enter the surrounding soil and confer multiple benefits to crops. Here, we used 1021, whose genome was sequenced in 2001, as a model bacterium to study the relationship between the bacterium and legume. We investigated the effects of hydrogen on the gene expression in using RNA-sequencing technology. We identified 43 genes whose expression was altered by hydrogen treatment; among these, 39 were downregulated, and 4 were upregulated. These genes accounted for 1.5% of the total 2941 annotated genes of the genome. Gene ontology and pathway analyses revealed that the hydrogen-regulated genes were associated with catalytic activity and binding. Further, these genes were primarily involved in arginine, proline, and β-alanine metabolism. Real-time PCR revealed that the transcription levels of , , , and were reduced after hydrogen treatment. These results provide a theoretical framework for exploring new metabolic pathways of . Hydrogen formed during nitrogen fixation in legumes can enter the surrounding soil and confer multiple benefits to crops. Here, we used 1021, whose genome was sequenced in 2001, as a model bacterium to study the relationship between the bacterium and legume. We investigated the effects of hydrogen on the gene expression in using RNA-sequencing technology. We identified 43 genes whose expression was altered by hydrogen treatment; among these, 39 were downregulated, and 4 were upregulated. These genes accounted for 1.5% of the total 2941 annotated genes of the genome. Gene ontology and pathway analyses revealed that the hydrogen-regulated genes were associated with catalytic activity and binding. Further, these genes were primarily involved in arginine, proline, and β-alanine metabolism. Real-time PCR revealed that the transcription levels of , , , and were reduced after hydrogen treatment. These results provide a theoretical framework for exploring new metabolic pathways of .
固氮过程中形成的氢气可以进入周围的土壤，并赋予作物多种益处。在这里，我们以 1021 为模式菌，对其与豆科植物的关系进行研究。1021 的基因组测序于 2001 年完成。我们使用 RNA 测序技术研究了氢气对 基因表达的影响。我们鉴定了 43 个受氢气处理影响的表达改变的基因；其中 39 个下调，4 个上调。这些基因占 基因组中 2941 个注释基因的 1.5%。基因本体论和途径分析表明，氢气调控的基因与催化活性和结合有关。此外，这些基因主要参与精氨酸、脯氨酸和β-丙氨酸代谢。实时 PCR 显示，氢气处理后 、 、 和 的转录水平降低。这些结果为探索 的新代谢途径提供了理论框架。
