Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, QLD, Australia.
Australian Infectious Disease Research Centre, School of Biological Sciences, The University of Queensland, Brisbane, QLD, Australia
J Virol. 2018 Aug 16;92(17). doi: 10.1128/JVI.00224-18. Print 2018 Sep 1.
Insect-specific viruses (ISVs) of the yellow fever mosquito have been demonstrated to modulate transmission of arboviruses such as dengue virus (DENV) and West Nile virus by the mosquito. The diversity and composition of the virome of , however, remains poorly understood. In this study, we characterized Aedes anphevirus (AeAV), a negative-sense RNA virus from the order AeAV identified from cell lines was infectious to both and cells but not to three mammalian cell lines. To understand the incidence and genetic diversity of AeAV, we assembled 17 coding-complete and two partial genomes of AeAV from available transcriptome sequencing (RNA-Seq) data. AeAV appears to transmit vertically and be present in laboratory colonies, wild-caught mosquitoes, and cell lines worldwide. Phylogenetic analysis of AeAV strains indicates that as the mosquito has expanded into the Americas and Asia-Pacific, AeAV has evolved into monophyletic African, American, and Asia-Pacific lineages. The endosymbiotic bacterium restricts positive-sense RNA viruses in Reanalysis of a small RNA library of cells coinfected with AeAV and produces an abundant RNA interference (RNAi) response consistent with persistent virus replication. We found enhances replication of AeAV compared to a tetracycline-cleared cell line, and AeAV modestly reduces DENV replication The results from our study improve understanding of the diversity and evolution of the virome of and adds to previous evidence that shows does not restrict a range of negative-strand RNA viruses. The mosquito transmits a number of arthropod-borne viruses (arboviruses), such as dengue virus and Zika virus. Mosquitoes also harbor insect-specific viruses that may affect replication of pathogenic arboviruses in their body. Currently, however, there are only a few insect-specific viruses described from in the literature. Here, we characterize a novel negative-strand virus, AeAV. Meta-analysis of samples showed that it is present in mosquitoes worldwide and is vertically transmitted. -transinfected mosquitoes are currently being used in biocontrol, as they effectively block transmission of several positive-sense RNA viruses in mosquitoes. Our results demonstrate that enhances the replication of AeAV and modestly reduces dengue virus replication in a cell line model. This study expands our understanding of the virome in as well as providing insight into the complexity of the virus restriction phenotype.
黄热病蚊的昆虫特异性病毒 (ISV) 已被证明可调节登革热病毒 (DENV) 和西尼罗河病毒等虫媒病毒在蚊子中的传播。然而， 的病毒组的多样性和组成仍知之甚少。在这项研究中，我们鉴定了黄病毒属 (AeAV)，这是一种来自 的负义 RNA 病毒。从细胞系中鉴定出的 AeAV 对 和 细胞具有感染性，但对三种哺乳动物细胞系没有感染性。为了了解 AeAV 的发病率和遗传多样性，我们从可用的转录组测序 (RNA-Seq) 数据中组装了 17 个编码完整和两个部分基因组的 AeAV。AeAV 似乎通过垂直传播存在于实验室种群、野外捕获的蚊子和世界各地的细胞系中。AeAV 菌株的系统发育分析表明，随着 蚊子扩展到美洲和亚太地区，AeAV 已进化为单系的非洲、美洲和亚太谱系。共生细菌 限制 中的正链 RNA 病毒 对 AeAV 和 的共感染细胞的小 RNA 文库的重新分析产生了丰富的 RNA 干扰 (RNAi) 反应，这与持续的病毒复制一致。我们发现 AeAV 的复制与四环素清除细胞系相比有所增强，并且 AeAV 适度降低了 DENV 的复制。我们的研究结果提高了对 病毒组多样性和进化的理解，并增加了之前的证据表明 不会限制一系列负链 RNA 病毒。蚊子 传播了许多节肢动物传播的病毒 (arboviruses)，如登革热病毒和寨卡病毒。蚊子还携带昆虫特异性病毒，这些病毒可能会影响其体内致病性 arboviruses 的复制。然而，目前文献中仅描述了几种从 中分离出的昆虫特异性病毒。在这里，我们描述了一种新型的负链病毒 AeAV。对 样本的元分析表明，它存在于世界各地的 蚊子中，并通过垂直传播。 -转染的蚊子目前正在被用于生物控制，因为它们有效地阻断了几种正链 RNA 病毒在蚊子中的传播。我们的结果表明，AeAV 在细胞系模型中增强了 AeAV 的复制，并适度降低了登革热病毒的复制。本研究扩展了我们对 病毒组的理解，并为 病毒限制表型的复杂性提供了见解。
