Department of Chemical, Biological, Pharmacological and Environmental Sciences, University of Messina, Messina, Italy.
Department of Biomedical and Dental Science and Morphofunctional Imaging, University of Messina, Messina, Italy.
Nat Prod Res. 2021 Nov;35(22):4454-4459. doi: 10.1080/14786419.2020.1727469. Epub 2020 Feb 18.
Morphological analysis and immunohistochemical determination of vitellogenin (Vtg) were carried out in zebrafish ovaries (). The goal was to determine the nutraceutical effect of Spirulina feed on ovarian maturation stages of adult zebrafish. After 30 d, in the stages of advanced maturation a prevalence of oocytes was found in the experimental group. Immunohistochemical investigations revealed a significant increase in Vtg, precursor of vitelline (Vn) in egg yolk, whose expression increased in the liver in relation to the stages of maturation. In the zebrafish liver, the expression of Vtg was extremely low before vitellogenesis, and then increased and it was maintained at a high level until the final stage of ovarian maturation. The results showed that food additives such as Spirulina can improve fertility in laboratory farmed fish. The data obtained from this study may be a contribution to wider research aimed at improving reproduction in all vertebrates, including humans.
对斑马鱼卵巢中的卵黄蛋白原（Vtg）进行形态分析和免疫组织化学测定。目的是确定螺旋藻饲料对成年斑马鱼卵巢成熟阶段的营养作用。30 天后，在成熟的后期阶段，实验组中发现卵母细胞的流行率。免疫组织化学研究表明，卵黄（Vn）的前体卵黄蛋白原（Vtg）在肝脏中的表达增加与成熟阶段有关。在斑马鱼肝脏中，卵黄发生前 Vtg 的表达极低，然后增加，并在卵巢成熟的最后阶段保持高水平。结果表明，螺旋藻等食品添加剂可以提高实验室养殖鱼类的繁殖力。本研究获得的数据可能有助于更广泛的研究，旨在提高所有脊椎动物（包括人类）的繁殖能力。
