妊娠早期口服[具体物质未给出]可调节诱导双胎母羊的产仔数以及抑制素、胰岛素、胰岛素样生长因子-I、辅酶Q10和骨形态发生蛋白-15的表达。
Oral Administration of at Early Gestation Modulates Litter Size and the Expression of Inhibin, Insulin, IGF-I, CO Q10, and BMP-15 in Ewes Induced for Twinning.
作者信息
Zeitoun Moustafa, Ali Mohamed, Almundarij Tariq, Mousa Emad, Alghonaim Ahmed
机构信息
Department of Animal Production and Breeding, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, Saudi Arabia.
Department of Animal and Fish Production, Faculty of Agriculture, Alexandria University, El-Shatby, Alexandria, Egypt.
出版信息
Vet Med Int. 2023 Jan 12;2023:7682533. doi: 10.1155/2023/7682533. eCollection 2023.
Gestation in sheep necessitates the support of nutrients to avoid early embryonic mortalities. Therefore, this study investigates the effects of supplementing either L-arginine or Spirulina alga in the first trimester on the pregnancy rate, litter size, inhibin, insulin, IGF-I, CO Q10, and bone morphogenetic peptide 15 (BMP15) in maternal circulation. Animals were offered barley (500 g/head/day, 14% CP), alfalfa hay (1 kg/head/day, 12% CP), clean water, and balanced salt block licks as free choices. Forty and ewes were randomly allotted into three groups: control (C, = 8), L-arginine (ARG, = 16), and Spirulina (SP, = 16). All females were implanted with CIDR for ten days. On days 8, 9, and 10, treated ewes were given a protocol comprised of human recombinant FSH at descending doses (50, 50, 40, 40, and 30, 30 IU, A.M, and P.M, respectively). At the fifth dose, animals were given an equivalent dose of hCG (240 IU). After CIDR withdrawal, ewes were exposed to fertile rams for mating. SP-ewes were orally given 50 ml (2%) Spirulina, and ARG-ewes were given 50 ml (35 mg/kg BW) L-arginine daily for 50 days postbreeding. Blood inhibin, insulin, IGF-I, CO Q10, and BMP15 were determined throughout gestation until parturition. The findings indicated that the conception rates were 25, 75, and 87.5% in C, ARG, and SP, respectively ( < 0.05). The percent of ewes giving birth to twins was 0, 25, and 50% in C, ARG, and SP, respectively ( < 0.05). The survival rates were 100, 81.8, and 83.3%, respectively. Birth weight was 5.6, 3.2, and 3.4 kg in C, ARG, and SP, respectively. Weaning weights were 28.3, 25.6, and 27.2 kg in C, ARG, and SP, respectively. BMP-15 was reduced ( < 0.05) in ARG than in C and SP. However, SP decreased ( < 0.05) inhibin more than in C and ARG. ARG and SP increased ( < 0.05) insulin than in C, whereas SP decreased ( < 0.05) IGF-I. SP increased CO Q10 compared with ARG. Ewes bearing twins revealed higher ( < 0.05) IGF-I (8.57 ng/ml) than those bearing singles (4.63 ng/ml); however, BMP-15 was higher in single (796.6 pg/ml) than in twin-bearing (387.5 pg/ml) ewes. In conclusion, providing early-gestating ewes with Spirulina enhances maternal health, productivity, and reproductive outcomes.
绵羊妊娠需要营养物质的支持以避免早期胚胎死亡。因此，本研究调查了在妊娠早期补充L-精氨酸或螺旋藻对母羊循环中妊娠率、产仔数、抑制素、胰岛素、胰岛素样生长因子-I（IGF-I）、辅酶Q10和骨形态发生蛋白15（BMP15）的影响。给动物提供大麦（500克/头/天，粗蛋白含量14%）、苜蓿干草（1千克/头/天，粗蛋白含量12%）、清洁饮水以及自由选择的平衡盐块舔砖。40只母羊被随机分为三组：对照组（C，n = 8）、L-精氨酸组（ARG，n = 16）和螺旋藻组（SP，n = 16）。所有母羊均植入孕酮释放阴道环（CIDR）10天。在第8、9和10天，对处理组母羊给予递减剂量的重组人促卵泡素方案（分别在上午和下午给予50、50、40、40和30、30国际单位）。在第5次给药时，给动物注射等量的人绒毛膜促性腺激素（hCG，240国际单位）。取出CIDR后，让母羊与可育公羊接触进行交配。繁殖后50天内，给SP组母羊每日口服50毫升（2%）螺旋藻，给ARG组母羊每日口服50毫升（35毫克/千克体重）L-精氨酸。在整个妊娠期直至分娩期间测定血液中的抑制素、胰岛素、IGF-I、辅酶Q10和BMP15。结果表明，C组、ARG组和SP组的受孕率分别为25%、75%和87.5%（P<0.05）。C组、ARG组和SP组产双羔的母羊百分比分别为0、25%和50%（P<0.05）。存活率分别为100%、81.8%和83.3%。C组、ARG组和SP组的出生体重分别为5.6千克、3.2千克和3.4千克。C组、ARG组和SP组的断奶体重分别为28.3千克、25.6千克和27.2千克。与C组和SP组相比，ARG组的BMP-15降低（P<0.05）。然而，与C组和ARG组相比，SP组的抑制素降低幅度更大（P<0.05）。与C组相比，ARG组和SP组的胰岛素升高（P<0.05），而SP组的IGF-I降低（P<0.05）。与ARG组相比，SP组的辅酶Q10升高。产双羔的母羊的IGF-I（8.57纳克/毫升）高于产单羔的母羊（4.63纳克/毫升）（P<0.05）；然而，产单羔母羊的BMP-15（796.6皮克/毫升）高于产双羔母羊（387.5皮克/毫升）。总之，给妊娠早期的母羊提供螺旋藻可改善母羊健康、提高生产性能和生殖结局。
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