Department of Chemical Engineering, Universidade Federal de São Paulo - UNIFESP, Diadema, São Paulo 09913-030, Brazil.
Department of Pharmaceutical Sciences, Universidade Federal de São Paulo - UNIFESP, Diadema, São Paulo 09913-030, Brazil.
Int J Biol Macromol. 2020 Jun 1;152:803-811. doi: 10.1016/j.ijbiomac.2020.02.140. Epub 2020 Feb 14.
In this study, we proposed the use of the biopolymers silk fibroin, chitosan and alginate, which are recognized for their biocompatibility and biodegradability, for the preparation of multilayer membranes aiming at high performance wound dressings with controlled drug delivery. The rationale was to combine in one material the mechanical properties of fibroin, the antimicrobial action of chitosan and the ideal exudate absorption of alginate, reaching a synergic effect of each biopolymer, without losing their individual intrinsic properties. The membranes were prepared by casting and diclofenac sodium was incorporated as model drug into the chitosan solution before the solvent evaporation, being retained in the middle layer of the membrane. Morphological, thermal, mechanical, solubility and barrier properties of the membranes were evaluated, as well as cytotoxicity and microbiological permeation. Results show that the incorporation of the drug did not affect mechanical and barrier properties, as well as microbiological permeation. Drug release was evaluated in vitro using simulated solution of wound exudate at 37 °C and diclofenac sodium was released from the multilayer membrane in 7 h, in which Fickian diffusion was the main mechanism associated. The results show the potential application of the biopolymer multilayer membranes as high-performance wound dressings.
在这项研究中，我们提出使用丝素蛋白、壳聚糖和海藻酸盐等生物聚合物，这些生物聚合物因其生物相容性和可生物降解性而得到认可，用于制备多层膜，旨在制备具有控释药物功能的高性能伤口敷料。其基本原理是将丝素蛋白的机械性能、壳聚糖的抗菌作用和海藻酸盐理想的渗出液吸收能力结合在一种材料中，达到每种生物聚合物的协同作用，同时不失去其各自的固有特性。通过浇铸法制备了这些膜，将双氯芬酸钠作为模型药物掺入壳聚糖溶液中，然后在溶剂蒸发前保留在膜的中间层。对膜的形态、热、机械、溶胀和阻隔性能进行了评价，同时还评价了细胞毒性和微生物渗透。结果表明，药物的掺入并不影响机械性能和阻隔性能，也不影响微生物渗透。在 37°C 的模拟伤口渗出液中进行了体外药物释放研究，双氯芬酸钠在 7 小时内从多层膜中释放出来，其中菲克扩散是主要的相关机制。研究结果表明，生物聚合物多层膜具有作为高性能伤口敷料的应用潜力。
