The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Oral Dis. 2020 Jul;26(5):974-982. doi: 10.1111/odi.13307. Epub 2020 Mar 4.
Irisin is a newly identified exercise-induced myokine which can affect glucose metabolism and cortical bone mass and strength. However, the influence of irisin on cementoblasts remains largely unknown.
An immortalized mouse cementoblast cell line OCCM-30 was used in this study. Cementoblast differentiation markers and PGC-1α in cells cultured with mineral induction medium were evaluated by qRT-PCR. Cementoblast mineralization was evaluated by alizarin red staining. Differentiation markers and the activity of p38 MAPK pathway under irisin stimulation were assessed by qRT-PCR or Western blot analysis. p38 MAPK pathway inhibitor SB203580 or p38 siRNA was used to further identify the regulatory mechanism. Cell proliferation treated with irisin was examined by CCK-8 method.
The expression of Runx2, osterix, ALP, and PGC-1α was up-regulated consistently under mineral induction. The formation of mineralized nodules was increased by irisin. Runx2, osterix, ALP, and osteocalcin were obviously up-regulated under irisin stimulation as well as the activity of p38 MAPK pathway. When pretreated with SB203580 or p38 siRNA before irisin stimulation, the irisin-induced differentiation was distinctly suppressed. OCCM-30 cell proliferation was enhanced when treated with high-dose irisin for long time.
Irisin can promote the differentiation of cementoblasts via p38 MAPK pathway.
鸢尾素是一种新发现的运动诱导肌因子，可影响葡萄糖代谢和皮质骨量和强度。然而，鸢尾素对成牙骨质细胞的影响在很大程度上尚不清楚。
本研究使用永生化的小鼠成牙骨质细胞系 OCCM-30。通过 qRT-PCR 评估细胞在矿化诱导培养基中培养时的成牙骨质细胞分化标志物和 PGC-1α。通过茜素红染色评估成牙骨质细胞的矿化。通过 qRT-PCR 或 Western blot 分析评估鸢尾素刺激下分化标志物和 p38 MAPK 通路的活性。使用 p38 MAPK 通路抑制剂 SB203580 或 p38 siRNA 进一步确定调节机制。通过 CCK-8 法检测鸢尾素处理后的细胞增殖。
在矿化诱导下，Runx2、osterix、ALP 和 PGC-1α 的表达一致上调。鸢尾素增加了矿化结节的形成。鸢尾素刺激下，Runx2、osterix、ALP 和骨钙素的表达明显上调，同时 p38 MAPK 通路的活性也增强。在鸢尾素刺激前用 SB203580 或 p38 siRNA 预处理，可明显抑制鸢尾素诱导的分化。长时间用高剂量鸢尾素处理可增强 OCCM-30 细胞的增殖。
鸢尾素可以通过 p38 MAPK 通路促进成牙骨质细胞的分化。
