Institute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, China.
Institute of Environmental Systems Biology, College of Environmental Science and Engineering, Dalian Maritime University, Dalian, 116026, China.
Environ Res. 2020 Mar;182:109123. doi: 10.1016/j.envres.2020.109123. Epub 2020 Jan 8.
Skatole is the key malodorous compound in livestock and poultry waste and wastewater with a low odor threshold. It not only causes serious nuisance to residents and workers, but also poses threat to the environment and human health due to its biotoxicity and recalcitrant nature. Biological treatment is an eco-friendly and cost-effective approach for skatole removal, while the bacterial resources are scarce. Herein, the Burkholderia strain was reported to efficiently degrade skatole for the first time. Results showed that strain IDO3 maintained high skatole-degrading performance under the conditions of pH 4.0-9.0, rotate speed 0-250 rpm, and temperature 30-35 °C. RNA-seq analysis indicated that skatole activated the oxidative phosphorylation and ATP production levels in strain IDO3. The oxidoreductase activity item which contained 373 differently expressed genes was significantly impacted by Gene Ontology analysis. Furthermore, the bioaugmentation experiment demonstrated that strain IDO3 could notably increase the removal of skatole in activated sludge systems. High-throughput 16S rRNA gene sequencing data indicated that the alpha-diversity and bacterial community tended to be stable in the bioaugmented group after 8 days operation. PICRUSt analysis indicated that xenobiotics biodegradation and metabolism, and membrane transport categories significantly increased, consistent with the improved skatole removal performance in the bioaugmented group. Burkholderia was survived and colonized to be the predominant population during the whole operation process (34.19-64.00%), confirming the feasibility of Burkholderia sp. IDO3 as the bioaugmentation agent in complex systems.
粪臭素是畜禽粪便和废水中的关键恶臭化合物，其气味阈值很低。它不仅对居民和工人造成严重滋扰，而且由于其生物毒性和难降解性，对环境和人类健康构成威胁。生物处理是去除粪臭素的一种环保且具有成本效益的方法，但细菌资源却很匮乏。本文首次报道了伯克霍尔德氏菌（Burkholderia）菌株能够有效地降解粪臭素。结果表明，IDO3 菌株在 pH 值为 4.0-9.0、转速为 0-250rpm 和温度为 30-35°C 的条件下，仍能保持较高的粪臭素降解性能。RNA-seq 分析表明，粪臭素激活了 IDO3 菌株的氧化磷酸化和 ATP 产生水平。基因本体论（GO）分析表明，氧化还原酶活性项目中包含的 373 个差异表达基因受到显著影响。此外，生物强化实验表明，IDO3 菌株能够显著提高活性污泥系统中粪臭素的去除率。高通量 16S rRNA 基因测序数据表明，经过 8 天的运行后，生物强化组的 alpha 多样性和细菌群落趋于稳定。PICRUSt 分析表明，外来化合物生物降解和代谢以及膜转运类群显著增加，与生物强化组中粪臭素去除性能的提高一致。伯克霍尔德氏菌在整个运行过程中存活并定殖为优势种群（34.19-64.00%），证实了 Burkholderia sp. IDO3 作为复杂系统生物强化剂的可行性。
