Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Ocean Science and Technology, Dalian University of Technology, Panjin 124221, China.
Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of Education), School of Ocean Science and Technology, Dalian University of Technology, Panjin 124221, China.
J Environ Sci (China). 2021 Mar;101:373-381. doi: 10.1016/j.jes.2020.09.002. Epub 2020 Sep 16.
Nitrogen-containing organic pollutants (quinoline, pyridine and indole) are widely distributed in coking wastewater, and bioaugmentation with specific microorganisms may enhance the removal of these recalcitrant pollutants. The bioaugmented system (group B) was constructed through inoculation of two aromatics-degrading bacteria, Comamonas sp. Z1 (quinoline degrader) and Acinetobacter sp. JW (indole degrader), into the activated sludge for treatment of quinoline, indole and pyridine, and the non-bioaugmented activated sludge was used as the control (group C). Both groups maintained high efficiencies (> 94%) for removal of nitrogen-containing organic pollutants and chemical oxygen demand (COD) during the long-term operation, and group B was highly effective at the starting period and the operation stage fed with raw wastewater. High-throughput sequencing analysis indicated that nitrogen-containing organic pollutants could shape the microbial community structure, and communities of bioaugmented group B were clearly separated from those of non-bioaugmented group C as observed in non-metric multidimensional scaling (NMDS) plot. Although the inoculants did not remain their dominance in group B, bioaugmentation could induce the formation of effective microbial community, and the indigenous microbes might play the key role in removal of nitrogen-containing organic pollutants, including Dokdonella, Comamonas and Pseudoxanthomonas. Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis suggested that bioaugmentation could facilitate the enrichment of functional genes related to xenobiotics biodegradation and metabolism, probably leading to the improved performance in group B. This study indicated that bioaugmentation could promote the removal of nitrogen-containing organic pollutants, which should be an effective strategy for wastewater treatment.
含氮有机污染物（喹啉、吡啶和吲哚）广泛分布于焦化废水中，通过接种特定的微生物进行生物强化可能会增强这些难降解污染物的去除效果。通过向活性污泥中接种两种芳烃降解菌——Comamonas sp. Z1（喹啉降解菌）和 Acinetobacter sp. JW（吲哚降解菌），构建了生物强化系统（B 组），并将非生物强化的活性污泥作为对照（C 组）用于处理喹啉、吲哚和吡啶。两组在长期运行过程中对含氮有机污染物和化学需氧量（COD）的去除均保持了很高的效率（>94%），且 B 组在初始阶段和处理原水的运行阶段效果非常显著。高通量测序分析表明，含氮有机污染物能够改变微生物群落结构，B 组的群落与非生物强化组 C 的群落明显分离，这一点在非度量多维尺度（NMDS）图中得到了体现。尽管接种剂在 B 组中没有保持优势地位，但生物强化可以诱导有效微生物群落的形成，土著微生物可能在去除含氮有机污染物方面发挥关键作用，包括 Dokdonella、Comamonas 和 Pseudoxanthomonas。群落构建的不可观测状态重建（PICRUSt）分析表明，生物强化可以促进与异生物质生物降解和代谢相关的功能基因的富集，这可能是 B 组性能提高的原因。本研究表明，生物强化可以促进含氮有机污染物的去除，这应该是一种有效的废水处理策略。
