Key Laboratory of Animal Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
Molecular and Cellular Biology, Gembloux Agro-Bio Tech, University of Liège, 5030 Gembloux, Belgium.
Int J Mol Sci. 2020 Feb 13;21(4):1248. doi: 10.3390/ijms21041248.
NCAPG is a subunit of condensin I that plays a crucial role in chromatin condensation during mitosis. has been demonstrated to be associated with farm animal growth traits. However, its role in regulating myoblast differentiation is still unclear. We used myoblasts derived from fetal bovine tissue as an in vitro model and found that was expressed during myogenic differentiation in the cytoplasm and nucleus. Silencing prolonged the mitosis and impaired the differentiation due to increased myoblast apoptosis. After 1.5 days of differentiation, silencing enhanced muscle-specific gene expression. An assay for transposase-accessible chromatin- high throughput sequencing (ATAC-seq) revealed that silencing altered chromatin accessibility to activating protein 1 (AP-1) and its subunits. Knocking down the expression of the AP-1 subunits fos-related antigen 2 ( or junB proto-oncogene enhanced part of the muscle-specific gene expression. In conclusion, our data provide valuable evidence about 's function in myogenesis, as well as its potential role in gene expression.
NCAPG 是凝缩蛋白 I 的亚基，在有丝分裂过程中对染色质凝聚起着至关重要的作用。 已被证明与家畜生长性状有关。然而，它在调节成肌细胞分化中的作用尚不清楚。我们使用来自胎牛组织的成肌细胞作为体外模型，发现 在成肌分化过程中在细胞质和核内表达。沉默 导致有丝分裂延长，并由于成肌细胞凋亡增加而损害分化。分化 1.5 天后，沉默增强了肌肉特异性基因的表达。转座酶可及染色质高通量测序 (ATAC-seq) 分析表明，沉默改变了激活蛋白 1 (AP-1)及其亚基的染色质可及性。敲低 AP-1 亚基 fos 相关抗原 2 ( 或 junB 原癌基因 的表达增强了部分肌肉特异性基因的表达。总之，我们的数据提供了关于 '在成肌发生中的功能以及其在基因表达中的潜在作用的有价值的证据。
