Department of Biology, New York University, New York 10003.
Center for Genomics and Systems Biology, New York University, New York 10003.
Genetics. 2018 Sep;210(1):331-344. doi: 10.1534/genetics.118.301217. Epub 2018 Jul 3.
Condensins are broadly conserved chromosome organizers that function in chromatin compaction and transcriptional regulation, but to what extent these two functions are linked has remained unclear. Here, we analyzed the effect of condensin inactivation on genome compaction and global gene expression in the yeast by performing spike-in-controlled genome-wide chromosome conformation capture (3C-seq) and mRNA-sequencing analysis. 3C-seq analysis shows that acute condensin inactivation leads to a global decrease in close-range intrachromosomal interactions as well as more specific losses of interchromosomal tRNA gene clustering. In addition, a condensin-rich interaction domain between the ribosomal DNA and the centromere on chromosome XII is lost upon condensin inactivation. Unexpectedly, these large-scale changes in chromosome architecture are not associated with global changes in mRNA levels. Our data suggest that the global transcriptional program of proliferating is resistant to condensin inactivation and the associated profound changes in genome organization.
凝缩素是广泛保守的染色体组织因子，在染色质紧缩和转录调控中发挥作用，但这两种功能在多大程度上相关尚不清楚。在这里，我们通过进行 Spike-in 控制的全基因组染色体构象捕获（3C-seq）和 mRNA 测序分析，分析了凝缩素失活对酵母 中基因组紧缩和全局基因表达的影响。3C-seq 分析表明，急性凝缩素失活导致近距离染色体内相互作用的整体减少，以及更具体的染色体间 tRNA 基因聚类的丧失。此外，在凝缩素失活时，位于染色体 XII 上的核糖体 DNA 和着丝粒之间的富含凝缩素的相互作用域也丢失了。出乎意料的是，这些染色体结构的大规模变化与 mRNA 水平的全局变化无关。我们的数据表明，增殖 的全局转录程序对凝缩素失活及其相关的基因组组织的深刻变化具有抗性。
