Poznan University of Life Sciences, Department of Food Biochemistry and Analysis, Mazowiecka 48, 60-623, Poznań, Poland.
Adam Mickiewicz University, Centre for Advance Technologies, Uniwersytetu Poznańskiego 10, 61-614, Poznań, Poland.
Talanta. 2020 May 1;211:120733. doi: 10.1016/j.talanta.2020.120733. Epub 2020 Jan 11.
Biochanin A is one of the most common phytoestrogens, occurring in high concentrations in soy and red clover, for instance, which shows a wide spectrum of biological activity. Prunetin is an isomer of biochanin A, and even though it is not very common, its structural relationship to the latter makes it interesting, regarding its biological activity. Nowadays, LC/ESI-MS methods are widely used for identification of natural compounds, including biochanin A and prunetin. However, we found that the published data concerning the identification of biochanin A and prunetin are sometimes disputable. Namely, the identification is based on the product ions which cannot be regarded as characteristic of biochanin A or prunetin. The reported retention times sometimes may be also disputable.
染料木黄酮是最常见的植物雌激素之一，大量存在于大豆和红三叶草等植物中，具有广泛的生物活性。樱黄素是染料木黄酮的异构体，虽然它并不常见，但由于它与后者在结构上的关系，使其在生物活性方面具有一定的研究意义。目前，LC/ESI-MS 方法广泛应用于天然化合物的鉴定，包括染料木黄酮和樱黄素。然而，我们发现有关染料木黄酮和樱黄素鉴定的已发表数据有时存在争议。具体来说，鉴定是基于不能被视为染料木黄酮或樱黄素特征的产物离子。报告的保留时间有时也可能存在争议。
