Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi Avenue, Durgapur 713209, West Bengal, India.
Department of Biotechnology, National Institute of Technology Durgapur, Mahatma Gandhi Avenue, Durgapur 713209, West Bengal, India.
Int J Biol Macromol. 2020 May 15;151:36-46. doi: 10.1016/j.ijbiomac.2020.02.112. Epub 2020 Feb 15.
Oxidative stress is considered to be the leading cause of many chronic and degenerative diseases leading to death and disability. Antioxidant therapy could be efficient in preventing oxidative stress-induced damages in cells. In the present study the efficacy of mangiferin-chitosan nanoparticles (MCNs) as a therapeutic agent against the oxidative stress, since mangiferin (MGF), a polyphenol protects and attenuates oxidative stress against various diseases. However, the bioactivity of MGF does not result in vivo biological effect owing to its low bioavailability and poor solubility. Mangiferin nanoparticles (NPs) were developed to improve the solubility and bioavailability of mangiferin. Nanoparticles had significant free radical scavenging activity in the cell-free system and had a synergistic action with phase II antioxidant enzymes such as catalases and peroxidases. A nephropathic system was developed to investigate the attenuative role of mangiferin-chitosan nanoparticles against induced oxidative stress, on normal kidney epithelial (NKE) cells. Pre-treatment with the nanoparticles exclusively prevented the induction of cytotoxicity induced by NaF and maintained the level of intracellular antioxidant enzyme in the cells. Nanoparticles had significant lipid peroxidation and protein oxidation inhibition activities. These nanoparticles can be used in food and pharmaceutical industries as a therapeutic agent to prevent the oxidative stress-induced health disorders.
氧化应激被认为是许多导致死亡和残疾的慢性和退行性疾病的主要原因。抗氧化治疗可能有效地预防细胞中氧化应激引起的损伤。在本研究中，研究了山竹堿-壳聚糖纳米粒（MCNs）作为一种治疗剂对抗氧化应激的功效，因为多酚山竹堿（MGF）可保护和减轻各种疾病引起的氧化应激。然而，由于其生物利用度低和溶解度差，MGF 的生物活性不会在体内产生生物学效应。开发山竹堿纳米粒（NPs）是为了提高山竹堿的溶解度和生物利用度。纳米粒在无细胞体系中具有显著的自由基清除活性，并与过氧化氢酶和过氧化物酶等 II 相抗氧化酶具有协同作用。建立了肾病系统来研究山竹堿-壳聚糖纳米粒对诱导氧化应激的衰减作用，以正常肾上皮（NKE）细胞为研究对象。纳米粒的预处理可单独预防 NaF 诱导的细胞毒性，并维持细胞内抗氧化酶的水平。纳米粒具有显著的抑制脂质过氧化和蛋白质氧化的活性。这些纳米粒可用于食品和制药行业作为治疗剂，以预防氧化应激引起的健康障碍。
