Harris A N, Perlman M, Schiller S L, Romero R, Mitchell M D
Department of Reproductive Medicine, University of California, San Diego, La Jolla.
Am J Obstet Gynecol. 1988 Dec;159(6):1385-9. doi: 10.1016/0002-9378(88)90561-3.
This study was undertaken to characterize prostaglandin production and its regulation in the human amnion-derived WISH cell line. Epidermal growth factor, tumor growth factor-alpha, tumor growth factor-beta, human interleukin-1, tumor necrosis factor, phorbol 12-myristate 13-acetate, phorbol 12,13-dibutyrate, 4 alpha-phorbol 12,13 didecanoate, and dexamethasone were tested for their ability to modulate prostaglandin production in WISH cells. Quantitatively, the major prostaglandin produced in WISH cells was prostaglandin E2. Treatment with epidermal growth factor, tumor growth factor-alpha, tumor necrosis factor, interleukin-1, phorbol 12,13-dibutyrate, and phorbol 12-myristate 13-acetate resulted in a concentration-dependent stimulation of WISH cell prostaglandin E2 production; tumor growth factor-beta and the inactive phorbol ester analog 4 alpha-phorbol 12,13 didecanoate had no effect. Dexamethasone treatment resulted in concentration-dependent inhibition of prostaglandin E2 production by WISH cells. WISH cells responded in a qualitatively similar manner to that previously observed in primary cultures of human amnion with the exception of the response to dexamethasone. On the basis of the findings of this investigation, we suggest that WISH cells may be a useful model for studying some but not all aspects of the regulation of arachidonic acid release and prostaglandin E2 formation in amnion. WISH cells may also be used to evaluate the mechanisms that link regulation of immune function and arachidonic acid metabolism.
本研究旨在描述人羊膜来源的WISH细胞系中前列腺素的产生及其调节。检测了表皮生长因子、肿瘤生长因子-α、肿瘤生长因子-β、人白细胞介素-1、肿瘤坏死因子、佛波醇12-肉豆蔻酸酯13-乙酸酯、佛波醇12,13-二丁酸酯、4α-佛波醇12,13-二癸酸酯和地塞米松调节WISH细胞中前列腺素产生的能力。从数量上看，WISH细胞产生的主要前列腺素是前列腺素E2。用表皮生长因子、肿瘤生长因子-α、肿瘤坏死因子、白细胞介素-1、佛波醇12,13-二丁酸酯和佛波醇12-肉豆蔻酸酯13-乙酸酯处理导致WISH细胞前列腺素E2产生呈浓度依赖性刺激；肿瘤生长因子-β和无活性的佛波醇酯类似物4α-佛波醇12,13-二癸酸酯无作用。地塞米松处理导致WISH细胞前列腺素E2产生呈浓度依赖性抑制。WISH细胞的反应在质量上与先前在人羊膜原代培养中观察到的相似，但对地塞米松的反应除外。基于本研究的结果，我们认为WISH细胞可能是研究羊膜中花生四烯酸释放和前列腺素E2形成调节的某些但不是所有方面的有用模型。WISH细胞也可用于评估连接免疫功能调节和花生四烯酸代谢的机制。
