Mota Ester Alves, Felestrino Érica Barbosa, Leão Versiane Albis, Guerra-Sá Renata
Biochemistry and Molecular Biology Laboratory, Department of Biological Sciences, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, Ouro Preto, MG, Brazil.
Bio & Hydrometallurgy Laboratory, Department of Metallurgical and Materials Engineering, Universidade Federal de Ouro Preto, Campus Morro do Cruzeiro, MG, Ouro Preto, Brazil.
Biotechnol Rep (Amst). 2020 Feb 4;25:e00431. doi: 10.1016/j.btre.2020.e00431. eCollection 2020 Mar.
Manganese (Mn) is toxic at higher concentrations requiring its removal before returning the wastewater to the environment. This article reported the Mn removal of two fungi strains isolated from mine wastewater. ITS rRNA region sequencing identified the fungi strains as and . Mn removal assays were performed in Sabouraud broth with 50 mg L Mn supplemented and bioleaching assays using MnO instead of MnSO at the same conditions. removed 96 % of 50 mg L Mn at two weeks without MnO bioleaching with 649.9 mg of biomass and removed about 50 % of Mn in 21 days from initial 50 mg of Mn L with 316.8 mg of biomass. Extracellular laccases were present in agar regardless of the Mn addition. Mn adsorbed was detected on hyphae. Mn oxidation was positive to by reaction of its medium with Leucoberbelin blue.
锰(Mn)在较高浓度时具有毒性,需要在废水排放回环境之前将其去除。本文报道了从矿山废水中分离出的两种真菌菌株对锰的去除情况。ITS rRNA区域测序将这两种真菌菌株鉴定为 和 。在添加了50 mg/L锰的沙氏肉汤中进行了锰去除试验,并在相同条件下使用MnO2代替MnSO4进行了生物浸出试验。在没有MnO2生物浸出的情况下,在两周内去除了50 mg/L锰的96%,生物量为649.9 mg;在21天内从初始的50 mg/L锰中去除了约50%,生物量为316.8 mg。无论是否添加锰,在 琼脂中都存在细胞外漆酶。在 菌丝上检测到了吸附的锰。通过其培养基与无色亚甲蓝反应,锰氧化对呈阳性。