Abat Deniz, Bayazıt Yıldırım, Açıkalın Arbil, Dağlıoğlu Kenan, Yenilmez Ebru Dündar, Altunkol Adem, Erdoğan Şeyda, Tuli Abdullah
İskenderun State Hospital, Department of Urology, Hatay, Turkey.
Ç ukurova University Faculty of Medicine, Department of Urology, Adana, Turkey.
J Pediatr Surg. 2018 Nov;53(11):2261-2265. doi: 10.1016/j.jpedsurg.2018.04.013. Epub 2018 Apr 14.
The aim of the study is to investigate the effect of Rolipram, a selective phosphodiesterase 4 inhibitor, on testicular torsion - detorsion injury.
Sixty young male rats were divided into five groups. In each group, the right testes of six rats were removed four hours after detorsion for biochemical analysis, and the right testes of the remaining six rats were removed 24 h after detorsion for pathological analysis. In group 1 (sham-operated) right orchiectomy was performed without torsion, and right testes were sent to the laboratory for biochemical and pathologic analyses. In group 2 (control) torsion was applied to the right testes for 60 min, and detorsion was performed without the administration of Rolipram. In group 3 torsion was applied to the right testes for 60 min. 1 mg/kg Rolipram was administered 30 min before detorsion. In group 4 torsion was applied to the right testes for 60 min, and 1 mg/kg Rolipram was administered during detorsion. In group 5 torsion was applied to the right testes for 60 min. 1 mg/kg Rolipram was administered 30 min after detorsion. The malondialdehyde and nitric oxide levels were determined. The rates of necrosis and apoptosis were evaluated by histopathological examination.
The level of malondialdehyde was higher in the torsioned groups (Group 2, 3, 4, 5) than that in group 1 (p = 0.004). There was no statistically significant difference between the groups regarding the level of nitric oxide (p = 0.182). Apoptosis was higher in groups 2, 3 and 4 than in group 1; however, apoptosis was similar in group 1 and group 5 (p = 0.122). The level of necrosis in group 1 was similar to that in groups 4 and 5 (p = 0.194 and p = 0.847, respectively).
We suggest that the administration of Rolipram can decrease the rate of necrosis and apoptosis in testicular ischaemia-reperfusion injury.
本研究旨在探讨选择性磷酸二酯酶4抑制剂咯利普兰对睾丸扭转-复位损伤的影响。
将60只年轻雄性大鼠分为五组。每组中，6只大鼠在复位后4小时切除右侧睾丸进行生化分析，其余6只大鼠在复位后24小时切除右侧睾丸进行病理分析。第1组（假手术组）行右侧睾丸切除术但不进行扭转，右侧睾丸送至实验室进行生化和病理分析。第2组（对照组）对右侧睾丸施加扭转60分钟，复位时不给予咯利普兰。第3组对右侧睾丸施加扭转60分钟，在复位前30分钟给予1mg/kg咯利普兰。第4组对右侧睾丸施加扭转60分钟，在复位过程中给予1mg/kg咯利普兰。第5组对右侧睾丸施加扭转60分钟，在复位后30分钟给予1mg/kg咯利普兰。测定丙二醛和一氧化氮水平。通过组织病理学检查评估坏死和凋亡率。
扭转组（第2、3、4、5组）的丙二醛水平高于第1组（p = 0.004）。各组之间一氧化氮水平无统计学显著差异（p = 0.182）。第2、3和4组的凋亡率高于第1组；然而，第1组和第5组的凋亡率相似（p = 0.122）。第1组的坏死水平与第4组和第5组相似（分别为p = 0.194和p = 0.847）。
我们认为，给予咯利普兰可降低睾丸缺血再灌注损伤中的坏死和凋亡率。
