Li Qin, Li Jing, Kang Ke-Lai, Wu Yi-Jun
Laboratory of Molecular Toxicology, State Key Laboratory for Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichenxilu Road, Beijing, 100101, People's Republic of China.
Environmental Standards Institute, Chinese Research Academy of Environmental Sciences, Beijing, 100012, People's Republic of China.
AMB Express. 2020 Feb 18;10(1):33. doi: 10.1186/s13568-020-00967-y.
Chemical pesticides are used widely and their residues are found in the environment. Pesticide pollution has become a global problem. To find an economical, effective and safety way to degrade residues of pesticides in environment, we constructed a genetically engineered bacterium (GEB) having the ability to degrade pesticides, emit green fluorescence and has a containment system by using a dual plasmid expression system. One plasmid contains the genes of enhanced green fluorescent protein (EGFP) and carboxylesterase B1 (CarE B1), which were cloned downstream of lambda P promoter and expressed constitutively. The gene of CarE B1 encodes an insect-detoxifying enzyme possessing the degradability to organochloride pesticides, organophosphorus pesticides, carbamates, and pyrethoid insecticides. The other is the conditional suicide plasmid for containment system, in which the lethal gene used was the nuclease gene of Serratia marcescens without the leader-coding sequence and was placed downstream of T7 promoter. The GEB has wide prospects of application on cleanup of pesticide residues with its degradability to several pesticides and containment system.
化学农药被广泛使用，其残留物存在于环境中。农药污染已成为一个全球性问题。为了找到一种经济、有效且安全的方法来降解环境中的农药残留，我们构建了一种基因工程菌（GEB），它具有降解农药、发出绿色荧光的能力，并通过使用双质粒表达系统拥有一个遏制系统。一个质粒包含增强型绿色荧光蛋白（EGFP）和羧酸酯酶B1（CarE B1）的基因，它们被克隆到λP启动子下游并组成型表达。CarE B1基因编码一种昆虫解毒酶，对有机氯农药、有机磷农药、氨基甲酸酯类和拟除虫菊酯类杀虫剂具有降解能力。另一个是用于遏制系统的条件自杀质粒，其中使用的致死基因是无前导编码序列的粘质沙雷氏菌核酸酶基因，它被置于T7启动子下游。这种基因工程菌因其对多种农药的降解能力和遏制系统，在农药残留清理方面具有广阔的应用前景。
