Zhang Chunyan, Zhang Huaran, Ju Jianhua
Institute of Medicinal Biotechnology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, Guangdong Key Laboratory of Marine Materia Medica, RNAM Center for Marine Microbiology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, 164 West Xingang Road, Guangzhou 510301, China.
Org Lett. 2020 Mar 6;22(5):1780-1784. doi: 10.1021/acs.orglett.0c00076. Epub 2020 Feb 19.
A silent ansamycin biosynthetic gene cluster () was activated in SCSIO T05 following mutagenesis and media optimization. A new shunt product, olimycin C () was produced by the -inactivated mutant strain, along with a minor product, olimycin D (). The production of these linear olimycin counterparts suggest that luciferase-like monooxygenase (LLM) OvmO catalyzes an on-PKS Baeyer-Villiger-type oxidation during assembly of the olimycin A () linear polyketide backbone.
一个沉默的安莎霉素生物合成基因簇在诱变和培养基优化后于中国科学院南海海洋研究所菌株T05中被激活。失活突变株产生了一种新的分流产物奥利霉素C，以及一种次要产物奥利霉素D。这些线性奥利霉素类似物的产生表明，类荧光素酶单加氧酶（LLM）OvmO在奥利霉素A线性聚酮骨架组装过程中催化非聚酮合酶（PKS）的拜耳-维利格型氧化反应。
