Key Laboratory of Pesticide and Chemical Biology (CCNU) Ministry of Education College of Chemistry, Central China Normal University, Wuhan, 430079, P.R. China.
Hubei Key Laboratory of Wudang Local Chinese Medicine Research Department of Chemistry School of Pharmacy, Hubei University of Medicine, Shiyan, 442000, Hubei Province, P. R. China.
Chem Asian J. 2020 Apr 1;15(7):1025-1029. doi: 10.1002/asia.201901821. Epub 2020 Mar 5.
In the life system, the biointerface plays an important role in cell adsorption, platelet adsorption and activation. Therefore, the study of protein adsorption on the biointerface is of great significance for understanding life phenomena and treatment in vitro. In this paper, a chiral biointerface was constructed by the virtue of host-guest interaction between a water-soluble pillar[5]arene (WP5) and phenethylamine (PEA) over a gold surface for adsorption of lysozyme proteins. From the experimental results it was identified that the host-guest biointerface has a high adsorption capacity and strong chiral selectivity. Furthermotre, it was identified that the host-guest interaction plays the decisive role in the enhancement of chirality of the interface, which was much beneficial for increasing protein adsorption and amplifying the capacity of chiral discrimination. Therefore, this work provides a new idea for the construction of biointerface materials with high protein adsorption capacity and high chiral selectivity through supramolecular interaction, which will have potential applications in the fields of biosensors, biocatalysts, biomaterials.
在生命体系中，生物界面在细胞吸附、血小板吸附和激活中起着重要作用。因此，研究蛋白质在生物界面上的吸附对于理解生命现象和体外治疗具有重要意义。本文利用水溶性杯[5]芳烃（WP5）与苯乙胺（PEA）在金表面上的主客体相互作用构建手性生物界面，用于溶菌酶蛋白的吸附。实验结果表明，主客体生物界面具有高吸附容量和强手性选择性。此外，研究发现主客体相互作用在手性增强中起决定性作用，这有利于增加蛋白质吸附并放大手性识别能力。因此，这项工作为通过超分子相互作用构建具有高蛋白质吸附能力和高手性选择性的生物界面材料提供了新的思路，在手性传感器、生物催化剂、生物材料等领域具有潜在的应用前景。
