Key Laboratory of Pesticide and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, P. R. China.
Department of Cardiology, The Third People's Hospital of Hubei Province Hospital of Hubei Province, Wuhan 430030, P. R. China.
Bioconjug Chem. 2022 Dec 21;33(12):2237-2244. doi: 10.1021/acs.bioconjchem.1c00527. Epub 2021 Dec 13.
In living systems, the adsorption of a protein on biointerfaces is a universal phenomenon, such as the specific binding of an antibody and antigen, which plays an important role in body growth and life maintenance. The exploration of a protein-selective adsorption on the biointerface is of great significance for understanding the life process and treatment in vitro. Herein, on the basis of biomimetic strategies, we fabricated a planar-chiral NH-pillar[5]arene modified silicon surface (R-/S-NP5 surfaces) for a highly enantioselective adsorption of protein by taking advantage of the guest-induced planar chirality of pillar[5]arenes. Results from practical experiments and theoretical calculations show that the R-NP5 surface possesses a high adsorption capacity and chiral selectivity for bovine serum albumin (BSA). Moreover, it was identified that the guest-induced chiral effect the generation and amplification of planar chirality, which was much beneficial for enhancing the interaction between planar-chiral pillar[5]arene host and BSA. The binding capacity of R-NP5 and BSA is stronger than that of S-NP5, thus promoting the chiral selective adsorption of BSA. This work affords a deeper understanding of the chiral influence of protein adsorption on biointerfaces and meanwhile provides a new perspective for chiral-sensing applications.
在生命系统中，蛋白质在生物界面上的吸附是一种普遍现象，例如抗体和抗原的特异性结合，这在身体生长和生命维持中起着重要作用。探索蛋白质在生物界面上的选择性吸附对于理解生命过程和体外治疗具有重要意义。在此基础上，我们利用杯[5]芳烃的客体诱导的平面手性，制备了一种平面手性 NH-柱[5]芳烃修饰的硅表面（R-/S-NP5 表面），用于蛋白质的高对映选择性吸附。实验和理论计算结果表明，R-NP5 表面对牛血清白蛋白（BSA）具有高吸附容量和手性选择性。此外，确定了客体诱导的手性效应对平面手性的产生和放大，这有利于增强平面手性杯[5]芳烃主体与 BSA 之间的相互作用。R-NP5 与 BSA 的结合能力强于 S-NP5，从而促进了 BSA 的手性选择性吸附。这项工作深入了解了蛋白质在生物界面上吸附的手性影响，同时为手性传感应用提供了新的视角。
