Batista Jaqueline Martins, Nakagaki Wilson Romero, Soares Evelise Aline, Camilli José Angelo
Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, Universidade Estadual de Campinas/UNICAMP, Programa de Pós-Graduação em Biologia Celular e Estrutural, Avenida Bertrand Russel, s/n, 13083-865 Campinas, SP, Brazil.
Programa de Mestrado em Ciências da Saúde, Universidade do Oeste Paulista/UNOESTE, Pró-Reitoria de Pesquisa e Pós-Graduação/Campus II, Rodovia Raposo Tavares, Km 572, Bairro do Limoeiro, 19067-175 Presidente Prudente, SP, Brazil.
An Acad Bras Cienc. 2020 Feb 17;92(1):e20180903. doi: 10.1590/0001-3765202020180903. eCollection 2020.
The periosteum is a rich source of osteoprogenitor cells and periosteal grafts can be used as an alternative method to replace bone grafts. The low-intensity pulsed ultrasound (LIPUS) has often been used as a noninvasive method to stimulate osteogenesis and reduce the fracture healing time. The aim of this study was to evaluate the effects of the ultrasound exposure on the rat tibia periosteum. Group I (7 animals) received LIPUS therapy on the left tibia for 7 days and group II (7 animals) on the left tibia for 14 days. After euthanasia, the tibias were processed. Number of periosteal cells and vessels and thickness of the periosteum were analyzed. The number of periosteal cells was higher in stimulated periosteum compared to controls at 7 and 14 days, but the number of vessels and the thickness only were higher in the group stimulated at 14 days. Furthermore, the ultrasound treatment for 14 days was more effective than 7 days. The ultrasound stimulation of the periosteum prior to grafting procedure can be advantageous, since it increases periosteal activity, and LIPUS may be an alternative method for stimulating the periosteum when the use of periosteal grafts in bone repair is needed.
骨膜是骨祖细胞的丰富来源，骨膜移植可作为替代骨移植的一种方法。低强度脉冲超声（LIPUS）常被用作一种非侵入性方法来刺激骨生成并缩短骨折愈合时间。本研究的目的是评估超声照射对大鼠胫骨骨膜的影响。第一组（7只动物）左侧胫骨接受LIPUS治疗7天，第二组（7只动物）左侧胫骨接受LIPUS治疗14天。安乐死后，对胫骨进行处理。分析骨膜细胞数量、血管数量和骨膜厚度。与对照组相比，在7天和14天时，受刺激骨膜中的骨膜细胞数量更高，但仅在14天受刺激组中血管数量和厚度更高。此外，超声治疗14天比7天更有效。在移植手术前对骨膜进行超声刺激可能是有利的，因为它会增加骨膜活性，并且当骨修复中需要使用骨膜移植时，LIPUS可能是刺激骨膜的一种替代方法。
