Forrester I T, Grabski A C, Burgess R R, Leatham G F
University of Wisconsin Biotechnology Center, Madison 53705.
Biochem Biophys Res Commun. 1988 Dec 30;157(3):992-9. doi: 10.1016/s0006-291x(88)80972-0.
Manganese and Mn-dependent peroxidases have been implicated in the enzymatic degradation of lignin. However, the specific role of manganese is uncertain. We report here the novel observation that in the absence of enzyme, suitably chelated Mn3+ is a ligninolytic agent capable of oxidizing veratryl alcohol, lignin model compounds, and lignin. We also demonstrate the unexpected effect of reducing agents which stimulate the oxidations by Mn3+. The stimulation is apparently through the production of a reduced oxygen species likely to be superoxide. These observations provide a fresh insight into the process of lignin biodegradation.
锰和锰依赖过氧化物酶与木质素的酶促降解有关。然而，锰的具体作用尚不确定。我们在此报告一项新的观察结果，即在没有酶的情况下，适当螯合的Mn3+是一种能够氧化藜芦醇、木质素模型化合物和木质素的木质素分解剂。我们还证明了还原剂对Mn3+氧化反应的意外促进作用。这种促进作用显然是通过产生一种可能是超氧化物的还原态氧物质实现的。这些观察结果为木质素生物降解过程提供了新的见解。
