Perez J, Jeffries T W
Institute for Microbial and Biochemical Technology, Forest Products Laboratory, Madison, WI 53705.
Appl Biochem Biotechnol. 1993 Spring;39-40:227-38. doi: 10.1007/BF02918992.
Nitrogen, carbon, and manganese are potent regulators of lignin degradation, but although nitrogen and carbon elicit a generalizated response when cells are starved, manganese is a relatively specific regulator of lignin and manganese peroxidase (LiP and MnP, respectively). At high manganese levels, MnP is induced, and LiP is repressed. At low Mn levels, MnP is repressed, and LiP is induced. Organic acid chelators are very important in attaining LiP repression with high Mn. Both mineralization and lignin depolymerization are regulated by manganese in the presence of organic acid chelators. As long as the chelators keep Mn(II) and Mn(III) in solution, repression is observed, but eventually, dismutation reactions cause the formation and precipitation of Mn (IV) as MnO2. Repression is immediately relieved, and depolymerization and mineralization proceed at a high rate.
氮、碳和锰是木质素降解的有效调节剂，但尽管当细胞饥饿时氮和碳会引发普遍反应，锰却是木质素和锰过氧化物酶（分别为LiP和MnP）相对特异性的调节剂。在高锰水平下，MnP被诱导，而LiP被抑制。在低锰水平下，MnP被抑制，而LiP被诱导。有机酸螯合剂对于在高锰情况下实现LiP抑制非常重要。在有机酸螯合剂存在的情况下，矿化和木质素解聚均受锰的调节。只要螯合剂使Mn(II)和Mn(III)保持在溶液中，就会观察到抑制作用，但最终，歧化反应会导致MnO2形式的Mn(IV)形成并沉淀。抑制作用立即解除，解聚和矿化以高速率进行。
