Podwin Agnieszka, Lizanets Danylo, Przystupski Dawid, Kubicki Wojciech, Śniadek Patrycja, Kulbacka Julita, Wymysłowski Artur, Walczak Rafał, Dziuban Jan A
Faculty of Microsystem Electronics and Photonics, Wrocław University of Science and Technology, Wrocław 50-370, Poland.
Department of Molecular and Cellular Biology, Faculty of Pharmacy, Wrocław Medical University, Wrocław 50-367, Poland.
Micromachines (Basel). 2020 Feb 14;11(2):196. doi: 10.3390/mi11020196.
This paper presents a full-featured microfluidic platform ensuring long-term culturing and behavioral analysis of the radically different biological micro-objects. The platform uses all-glass lab-chips and MEMS-based components providing dedicated micro-aquatic habitats for the cells, as well as their intentional disturbances on-chip. Specially developed software was implemented to characterize the micro-objects metrologically in terms of population growth and cells' size, shape, or migration activity. To date, the platform has been successfully applied for the culturing of freshwater microorganisms, fungi, cancer cells, and animal oocytes, showing their notable population growth, high mobility, and taxis mechanisms. For instance, circa 100% expansion of porcine oocytes cells, as well as nearly five-fold increase in population, has been achieved. These results are a good base to conduct further research on the platform versatile applications.
本文展示了一个功能齐全的微流控平台，可确保对截然不同的生物微对象进行长期培养和行为分析。该平台使用全玻璃实验室芯片和基于MEMS的组件，为细胞提供专门的微水生栖息地，以及在芯片上对其进行有意干扰。实施了专门开发的软件，以便从种群增长以及细胞的大小、形状或迁移活动方面对微对象进行计量表征。迄今为止，该平台已成功应用于淡水微生物、真菌、癌细胞和动物卵母细胞的培养，显示出它们显著的种群增长、高迁移率和趋化机制。例如，猪卵母细胞实现了约100%的扩增，种群数量增加了近五倍。这些结果是对该平台多功能应用进行进一步研究的良好基础。
