Graduate School of Life and Environmental Sciences, Azabu University, Kanagawa 252-5201, Japan.
Department of Computer Science, Tokyo Institute of Technology, Tokyo 152-8552, Japan.
Toxins (Basel). 2020 Feb 15;12(2):125. doi: 10.3390/toxins12020125.
Citreoviridin (CTV) is a mycotoxin that is produced by , and , and CTV has been detected in a wide range of cereal grains throughout the world. Furthermore, it is especially a serious problem in regions where rice is consumed as a staple food. Moreover, CTV is a well-known yellow rice toxin, and outbreaks of beriberi have occurred due to consumption of rice that is contaminated by CTV even in the recent years. Although CTV biosynthetic genes of have been described, those of remain unclear, which is concerning since is the main cause of CTV contamination in rice. In the present study, we determined the draft genome of the strain IMI92228 and revealed the presence of all four genes that form a gene cluster and that are homologous to the CTV biosynthesis genes of . The expression of these four homologous genes were highly correlated with CTV production, suggesting that they may play an important role in CTV biosynthesis in . We concluded that the gene cluster is a CTV biosynthesis cluster of . The findings will contribute to the understanding of the biosynthetic pathway of CTV and will ultimately lead to improvements in the CTV management of agricultural products.
桔青霉素（CT V）是一种真菌毒素，由 和 产生，已在世界各地的各种谷物中检测到。此外，在以大米为主食的地区，CT V 是一个特别严重的问题。此外，CT V 是一种众所周知的黄米毒素，即使在近几年，由于食用被 CTV 污染的大米，脚气病也曾爆发过。虽然 的 CTV 生物合成基因已被描述，但 的基因仍不清楚，这令人担忧，因为 是大米中 CTV 污染的主要原因。在本研究中，我们确定了 IMI92228 菌株的草图基因组，并揭示了存在形成基因簇的所有四个基因，这些基因与 的 CTV 生物合成基因同源。这四个同源基因的表达与 CTV 的产生高度相关，表明它们可能在 中 CTV 的生物合成中发挥重要作用。我们得出结论，该基因簇是 的 CTV 生物合成簇。这些发现将有助于了解 CTV 的生物合成途径，并最终导致农产品中 CTV 管理的改进。
