Wellcome Sanger Institute, Hinxton, Cambridgeshire CB10 1SA, United Kingdom; email:
Theory of Condensed Matter, Department of Physics, University of Cambridge, Cambridgeshire CB3 0HE, United Kingdom.
Annu Rev Immunol. 2020 Apr 26;38:727-757. doi: 10.1146/annurev-immunol-090419-020340. Epub 2020 Feb 19.
Immune cells are characterized by diversity, specificity, plasticity, and adaptability-properties that enable them to contribute to homeostasis and respond specifically and dynamically to the many threats encountered by the body. Single-cell technologies, including the assessment of transcriptomics, genomics, and proteomics at the level of individual cells, are ideally suited to studying these properties of immune cells. In this review we discuss the benefits of adopting single-cell approaches in studying underappreciated qualities of immune cells and highlight examples where these technologies have been critical to advancing our understanding of the immune system in health and disease.
免疫细胞的特征包括多样性、特异性、可塑性和适应性——这些特性使它们能够为体内的稳态做出贡献，并对身体遇到的许多威胁做出特异性和动态的反应。单细胞技术，包括对单个细胞水平的转录组学、基因组学和蛋白质组学的评估，非常适合研究免疫细胞的这些特性。在这篇综述中，我们讨论了采用单细胞方法来研究免疫细胞被低估的特性的好处，并强调了这些技术在推进我们对健康和疾病中免疫系统的理解方面至关重要的例子。
