Biofilm Research Laboratory, Faculty of Dental Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel.
The Institute for Drug Research, School of Pharmacy, The Hebrew University of Jerusalem, Jerusalem, Israel.
Biomed Res Int. 2020 Jan 31;2020:7258380. doi: 10.1155/2020/7258380. eCollection 2020.
Endocannabinoid/endocannabinoid-like (EC/EC-like) are natural endogenous compounds which have been found to affect MRSA pathogenicity. Our previous studies showed that EC/EC-like was able to impair staphylococcal biofilm formation and maintenance as well as to alter biofilm-associated virulence factors. In the present study, we investigated the combinatory effect of the selected EC/EC-like with a natural antimicrobial agent, poly-L-lysine, on cariogenic bacteria growth and biofilm formation. Among four tested EC/EC-like, only two, anandamide (AEA) and oleoylethanolamide (OEA), exhibited synergistic combinatory effect with poly-L-lysine against . . We attribute this distinct effect to differences in the fatty acid chain structure of the selected EC/EC-like compounds. Moreover, AEA exerted a specific antibiofilm mode of action against . by effecting total inhibition of biofilm formation while still allowing bacteria viability. Finally, we postulate that the presence of EC/EC-like and poly-L-lysine could enhance the permeability and efficacy of each other via hydrophobic and electrostatic interactions with the . membrane. In conclusion, we assume that a combination of endogenous natural compounds such as EC/EC-like and poly-L-lysine may benefit oral hygiene by preventing dental plaque.
内源性大麻素/内源性大麻素样物质（EC/EC 样）是天然的内源性化合物，已被发现会影响耐甲氧西林金黄色葡萄球菌的致病性。我们之前的研究表明，EC/EC 样物质能够损害金黄色葡萄球菌生物膜的形成和维持，并改变生物膜相关的毒力因子。在本研究中，我们研究了选定的 EC/EC 样物质与天然抗菌剂聚赖氨酸联合对致龋菌生长和生物膜形成的组合效应。在测试的四种 EC/EC 样物质中，只有两种，即花生四烯酸乙醇胺（AEA）和油酰乙醇胺（OEA），与聚赖氨酸对 表现出协同的组合效应。我们将这种明显的效果归因于所选 EC/EC 样物质的脂肪酸链结构的差异。此外，AEA 通过完全抑制生物膜形成对 发挥特定的抗生物膜作用，同时仍允许细菌存活。最后，我们假设 EC/EC 样物质和聚赖氨酸的存在可以通过与 细胞膜的疏水和静电相互作用来增强彼此的通透性和功效。总之，我们假设内源性天然化合物（如 EC/EC 样物质和聚赖氨酸）的组合可能通过预防牙菌斑来有益于口腔卫生。
