Liu Xu, Meng Lei, Song Wenzhu, Zhi Mengfan, Wang Peiyu, Liu Bin, Du Mi, Feng Qiang
Department of Human Microbiome & Implantology, School and Hospital of Stomatology, Cheeloo College of Medicine, Shandong University & Shandong Key Laboratory of Oral Tissue Regeneration & Shandong Engineering Laboratory for Dental Materials and Oral Tissue Regeneration & Shandong Provincial Clinical Research Center for Oral Diseases, Jinan, China.
Shandong University-BOP Joint Oral Microbiome Laboratory, Jinan, China.
Int Dent J. 2025 Apr;75(2):1068-1080. doi: 10.1016/j.identj.2024.11.017. Epub 2025 Jan 13.
To evaluate the effect of the toothpaste containing ε-poly-L-lysine (ε-PL) and funme peptide (FP) as key components on oral microbial composition and oral health.
An oral microbiome study was initially carried out to analyze the variation in the oral microbiota before and after use of antimicrobial peptide (AMP) toothpaste. Subsequently, a clinical trial was independently performed to assess the efficacy of AMP toothpaste by measuring the dental plaque index (PLI), volatile sulfur compounds (VSCs) levels, modified bleeding index (mBI), and bleeding on probing rate (BOP%).
The application of AMP toothpaste increased the α diversity and modified β diversity of oral microbiome across 3 oral niches. AMP toothpaste increased the relative abundance of the commensal oral microbes, and attenuated the abundance of pathogenic bacteria in gingivitis patients to normal levels. The clinical trial showed 44.33% and 12.29% reductions of PLI scores in the test and control groups, respectively, and the test group exhibited a more pronounced decrease in VSC levels. The test group recorded significant reductions in mBI and BOP% by 39.09% and 24.59%, respectively, exceeding the control group's reductions of 4.63% and -0.97% (P < .05).
The formulation of toothpaste with ε-PL and FP recalibrated the oral microbiome's diversity and abundance, and mitigated common oral health issues such as plaque, halitosis, and gingivitis while maintaining well safety.
Oral care products containing ε-PL and FP can be used as a new treatment for improving oral microbiota and oral diseases.
评估以ε-聚-L-赖氨酸（ε-PL）和富美肽（FP）为关键成分的牙膏对口腔微生物组成和口腔健康的影响。
最初开展了一项口腔微生物组研究，以分析使用抗菌肽（AMP）牙膏前后口腔微生物群的变化。随后，独立进行了一项临床试验，通过测量牙菌斑指数（PLI）、挥发性硫化合物（VSC）水平、改良出血指数（mBI）和探诊出血率（BOP%）来评估AMP牙膏的疗效。
AMP牙膏的使用增加了3个口腔部位口腔微生物组的α多样性并改变了β多样性。AMP牙膏增加了口腔共生微生物的相对丰度，并将牙龈炎患者体内的病原菌丰度降至正常水平。临床试验显示，试验组和对照组的PLI评分分别降低了44.33%和12.29%，且试验组的VSC水平下降更为明显。试验组的mBI和BOP%分别显著降低了39.09%和24.59%，超过了对照组4.63%和-0.97%的降幅（P<.05）。
含有ε-PL和FP的牙膏配方重新校准了口腔微生物组的多样性和丰度，减轻了牙菌斑、口臭和牙龈炎等常见口腔健康问题，同时保持了良好的安全性。
含有ε-PL和FP的口腔护理产品可作为改善口腔微生物群和口腔疾病的新疗法。
