Post-Doctoral Research Center of Chongqing Key Laboratory of Inorganic Special Functional Materials, College of Chemistry and Chemical Engineering, Yangtze Normal University, Fuling, Chongqing, 408100, China.
School of Materials Science and Engineering, College of Chemical and Environmental Engineering, Harbin University of Science and Technology, Harbin, 150040, Heilongjiang, China.
Anal Bioanal Chem. 2020 Apr;412(11):2391-2397. doi: 10.1007/s00216-020-02481-3. Epub 2020 Feb 19.
By adding 6 thymines to lengthen the parent aptamer combined with the change of "on" and "off" induced by the target for an assistant stem-loop DNA probe (ASP-SLP-MB), a new folding-type electrochemical kanamycin (Kana) aptamer-engineering dual-probe-based sensor (sensor d) was developed. By purposefully reducing the background current and increasing the electron transfer efficiency of methylene blue (MB), the sensor obtained significantly enhanced detection sensitivity compared with non-aptamer-engineering one-probe-based sensor (sensor a). Such efficacy was validated by a big decrease from 530.6 to 210.2 nA for the background current signal and from 360 to 0.3 nM for the detection limit. In addition to the improved sensitivity, the sensor also exhibited good selectivity, anti-fouling detection performance, and potential quantitative analysis ability, showing a feasible potential practical analytical application in real-life complicated samples, for example, milk and serum. The released results prove that the aptamer-engineering method is effective in improving the analytical performance of folding-type sensors and provides a methodological guidance for the design and fabrication of other high-performance folding-type aptasensors. Graphical abstract.
通过在亲本适体上添加 6 个胸腺嘧啶以延长其长度，并结合目标物诱导的“开”和“关”变化，设计了一种新型折叠型电化学卡那霉素（Kana）适体工程双探针传感器（传感器 d）。通过有目的地降低背景电流并提高亚甲基蓝（MB）的电子转移效率，与非适体工程单探针传感器（传感器 a）相比，该传感器获得了显著增强的检测灵敏度。通过背景电流信号从 530.6 降至 210.2 nA，检测限从 360 降至 0.3 nM，验证了这种效果。除了提高的灵敏度外，该传感器还表现出良好的选择性、抗污染检测性能和潜在的定量分析能力，在真实复杂样品（例如牛奶和血清）中显示出可行的潜在实际分析应用。已发布的结果证明，适体工程方法可有效提高折叠型传感器的分析性能，并为其他高性能折叠型适体传感器的设计和制造提供方法学指导。
