School of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu, China.
Anal Bioanal Chem. 2020 Apr;412(11):2487-2494. doi: 10.1007/s00216-020-02470-6. Epub 2020 Feb 19.
A versatile nanocomposite was simply prepared based upon the electrostatic adsorption of positively charged gold nanoparticles with negatively charged graphene oxide (nano-gold@GO), and utilized as a novel fluorescence quenching platform for ultrasensitive detection of adenosine triphosphate (ATP). In the designed system, DNA-stabilized Ag nanoclusters (DNA/AgNCs) were used as fluorescent probes, DNA duplex was formed in the presence of ATP, and they can electrostatically adsorb onto the surface of nano-gold@GO to quench the fluorescence signal. Upon the addition of exonuclease III (Exo III), the DNA duplex would be hydrolyzed into DNA fragments and resulted in the recovery of the fluorescence signals due to the diffusion of AgNCs away from nano-gold@GO. Based on these, sensitive detection of ATP was realized with a detection range of 5.0 pM-20 nM. Notably, a good recovery in the range of 94-104% was obtained when detecting ATP in human serum samples, indicating a promising application value in early disease diagnosis. Graphical abstract A functional positively charged nano-gold@graphene oxide was fabricated and utilized as an enhanced fluorescence quenching platform for the detection of ATP, coupled with exonuclease III-assisted signal amplification.
一种基于带正电荷的金纳米粒子与带负电荷的氧化石墨烯（纳米金@GO）静电吸附的多功能纳米复合材料被简单制备，并被用作一种新型荧光猝灭平台，用于超灵敏检测三磷酸腺苷（ATP）。在设计的系统中，DNA 稳定的银纳米团簇（DNA/AgNCs）被用作荧光探针，在存在 ATP 的情况下形成 DNA 双链体，它们可以静电吸附在纳米金@GO 的表面上，从而猝灭荧光信号。加入外切酶 III（Exo III）后，DNA 双链体将被水解成 DNA 片段，并由于 AgNCs 从纳米金@GO 上扩散而恢复荧光信号。基于这些，通过检测 5.0 pM-20 nM 的范围实现了对 ATP 的敏感检测。值得注意的是，在检测人血清样品中的 ATP 时，获得了 94-104%的良好回收率，表明其在早期疾病诊断中有很好的应用价值。
