用于可视化人胶质瘤组织中单胺氧化酶 A 活性的双光子荧光探针的合理设计。
Rational Design of a Two-Photon Fluorogenic Probe for Visualizing Monoamine Oxidase A Activity in Human Glioma Tissues.
机构信息
Key Laboratory of Flexible Electronics (KLOFE) & Institute of Advanced Materials (IAM), Nanjing Tech University (NanjingTech), 30 South Puzhu Road, Nanjing, 211816, P. R. China.
Department of Burns and Cutaneous Surgery, Xijing Hospital, The Fourth Military Medical University, Xi'an, 710032, P. R. China.
出版信息
Angew Chem Int Ed Engl. 2020 May 4;59(19):7536-7541. doi: 10.1002/anie.202000059. Epub 2020 Mar 13.
Monoamine oxidases have two functionally distinct but structurally similar isoforms (MAO-A and MAO-B). The ability to differentiate them by using fluorescence detection/imaging technology is of significant biological relevance, but highly challenging with available chemical tools. Herein, we report the first MAO-A-specific two-photon fluorogenic probe (F1), capable of selective imaging of endogenous MAO-A enzymatic activities from a variety of biological samples, including MAO-A-expressing neuronal SY-SY5Y cells, the brain of tumor-bearing mice and human Glioma tissues by using two-photon fluorescence microscopy (TPFM) with minimal cytotoxicity.
单胺氧化酶有两种功能不同但结构相似的同工酶（MAO-A 和 MAO-B）。使用荧光检测/成像技术将它们区分开来的能力具有重要的生物学意义，但现有的化学工具对此极具挑战性。在此，我们报告了第一个 MAO-A 特异性的双光子荧光探针（F1），它能够通过双光子荧光显微镜（TPFM）选择性地对各种生物样品中的内源性 MAO-A 酶活性进行成像，包括表达 MAO-A 的神经元 SY-SY5Y 细胞、荷瘤小鼠的大脑和人神经胶质瘤组织，且细胞毒性最小。
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