通过基因组挖掘发现并表征中的一个 PKS-NRPS 杂合酶。

Discovery and Characterization of a PKS-NRPS Hybrid in by Genome Mining.

机构信息

College of Bioscience and Bioengineering, Jiangxi Engineering Laboratory for the Development and Utilization of Agricultural Microbial Resources , Jiangxi Agricultural University , Nanchang 330045 , People's Republic of China.

Shandong Provincial Key Laboratory of Synthetic Biology , Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences , No. 189 Songling Road , Qingdao 266101 , People's Republic of China.

出版信息

J Nat Prod. 2020 Feb 28;83(2):473-480. doi: 10.1021/acs.jnatprod.9b01140. Epub 2020 Feb 20.

DOI:10.1021/acs.jnatprod.9b01140

PMID:32077283

Abstract

Fungal polyketide synthase-nonribosomal peptide synthetase (PKS-NRPS) hybrids have been characterized to produce polyketide-amino acid compounds with striking structural features and biological activities. In this study, a PKS-NRPS hybrid enzyme was found in by genome mining. By activating the cluster-specific transcriptional regulator, this cryptic PKS-NRPS gene cluster was successfully activated and ten products (-) were identified as pyranterreones. Using functional genetics, bioinformatics, and isotope-labeling feeding analysis, the biosynthetic pathway was revealed. This is the second PKS-NRPS hybrid identified in .

摘要

真菌聚酮合酶-非核糖体肽合酶（PKS-NRPS）杂合子已被证实能够产生具有显著结构特征和生物活性的聚酮-氨基酸化合物。在本研究中，通过基因组挖掘，在中发现了一种 PKS-NRPS 杂合酶。通过激活簇特异性转录调节因子，成功激活了这个隐藏的 PKS-NRPS 基因簇，并鉴定出十个产物（-）为吡喃替雷酮。利用功能遗传学、生物信息学和同位素标记喂养分析揭示了其生物合成途径。这是在中鉴定出的第二种 PKS-NRPS 杂合酶。