Department of Natural Sciences, Middlesex University London, London, UK.
Soil and Water Department, University of Florida, Gainesville, FL, USA.
J Sci Food Agric. 2020 May;100(7):3078-3086. doi: 10.1002/jsfa.10340. Epub 2020 Mar 2.
Nitric oxide (NO) donors have been used to control biofilm formation. Nitric oxide can be delivered in situ using organic carriers and acts as a signaling molecule. Cells exposed to NO shift from biofilm to the planktonic state and are better exposed to the action of disinfectants. In this study, we investigate the capability of the NO donors molsidomine, MAHAMA NONOate, NO-aspirin and diethylamine NONOate to act as anti-adhesion agents on ready-to-eat vegetables, as well as dispersants for a number of pathogenic biofilms on plastic.
Our results showed that 10 pM molsidomine reduced the attachment of Salmonella enterica sv Typhimurium 14 028 to pea shoots and coriander leaves of about 0.5 Log(CFU/leaf) when compared with untreated control. The association of 10 pmol L molsidomine with 0.006% H O showed a synergistic effect, leading to a significant reduction in cell collection on the surface of the vegetable of about 1 Log(CFU/leaf). Similar results were obtained for MAHMA NONOate. We also showed that the association of diethylamine NONOate at 10 mmol L and 10 pmol L with the quaternary ammonium compound diquat bromide improved the effectiveness of biofilm dispersal by 50% when compared with the donor alone.
Our findings reveal a dual role of NO compounds in biofilm control. Molsidomine, MAHMA NONOate, and diethylamine NONOate are good candidates for either preventing biofilm formation or dispersing biofilm, especially when used in conjunction with disinfectants. Nitric oxide compounds have the potential to be developed into a toolkit for pro-active practices for good agricultural practices (GAPs), hazard analysis and critical control points (HACCP), and cleaning-in-place (CIP) protocols in industrial settings where washing is routinely applied. © 2020 Society of Chemical Industry.
一氧化氮（NO）供体已被用于控制生物膜形成。NO 可以通过有机载体原位输送，并作为信号分子发挥作用。暴露于 NO 的细胞从生物膜转变为浮游状态，并且更容易受到消毒剂的作用。在这项研究中，我们研究了 NO 供体吗多明、MAHAMA NONOate、NO-阿司匹林和二乙胺 NONOate 作为即食蔬菜的抗粘附剂的能力，以及对塑料上多种致病性生物膜的分散剂的能力。
我们的结果表明，与未经处理的对照相比，10 pM 吗多明可将鼠伤寒沙门氏菌 14028 附着在豌豆苗和香菜叶上的数量减少约 0.5 Log(CFU/叶)。10 pmol L 吗多明与 0.006%H2O2的联合使用表现出协同作用，导致蔬菜表面细胞收集量显著减少约 1 Log(CFU/叶)。MAHMA NONOate 也得到了类似的结果。我们还表明，当与季铵化合物二氯溴化二喹啉联合使用时，二乙胺 NONOate 在 10 mmol L 和 10 pmol L 下的联合使用可将生物膜分散的有效性提高 50%，而与供体单独使用相比。
我们的研究结果揭示了 NO 化合物在生物膜控制中的双重作用。吗多明、MAHMA NONOate 和二乙胺 NONOate 是预防生物膜形成或分散生物膜的良好候选物，特别是在与消毒剂联合使用时。一氧化氮化合物有可能被开发成一种工具包，用于主动实践良好农业规范（GAPs）、危害分析和关键控制点（HACCP）和工业清洗（CIP）协议，在这些领域中，洗涤是常规应用的。 © 2020 英国化学学会。
