Gander I, Foeckler R, Rogge L, Meisterernst M, Schneider R, Mertz R, Lottspeich F, Winnacker E L
Institut für Biochemie der Universität München, F.R.G.
Biochim Biophys Acta. 1988 Dec 20;951(2-3):411-8. doi: 10.1016/0167-4781(88)90114-5.
The paper describes a potent purification method, preparative gel retention, for the purification of sequence-specific DNA-binding proteins. This procedure exploits the sequence-specific DNA-binding affinity of such proteins for their enrichment, comparable to recognition site DNA affinity chromatography. The method was employed to obtain a pure preparation of nuclear factor I (NFI) from porcine liver from which sequences of partial peptides could be obtained. Oligonucleotide probes derived from these amino-acid sequences were used to identify genomic and cDNA clones of NFI.
本文描述了一种用于纯化序列特异性DNA结合蛋白的高效纯化方法——制备性凝胶保留法。该方法利用此类蛋白质的序列特异性DNA结合亲和力来实现富集，类似于识别位点DNA亲和色谱法。该方法被用于从猪肝中获得核因子I（NFI）的纯制剂，从中可以获得部分肽段的序列。源自这些氨基酸序列的寡核苷酸探针被用于鉴定NFI的基因组和cDNA克隆。
