Department of Clinical Laboratory, Mianyang Central Hospital, Mianyang, Sichuan, China.
Department of Pharmacy, Sichuan Cancer Hospital and Institute, Sichuan Cancer Center, School of Medicine, University of Electronic Science and Technology of China, Chengdu, Sichuan, China.
Scand J Clin Lab Invest. 2020 May;80(3):247-255. doi: 10.1080/00365513.2020.1729401. Epub 2020 Feb 20.
Few studies were reported on trace elements' alterations in uremic patients undergoing long-term (>24 months) hemodialysis (HD), especially by using the whole blood as the biological fluid for the measuring purpose. Our objective was to develop an improved micro-sampling inductively coupled plasma-mass spectrometry (ICP-MS) method to determine the levels of Ca, Mg, Cu, Zn, Fe, Mn, Se and Pb in uremic patients receiving long-term HD. A ICP-MS method with a modified whole blood sample preparation procedure with small volumes was established and applied for the simultaneous quantification of the various elements in uremic patients undergoing long-term HD. 124 eligible uremic patients receiving long-term HD (75 males and 49 females) and 77 healthy subjects (54 males and 23 females) were recruited and Ca, Mg, Cu, Zn, Fe, Mn, Se and Pb levels were further determined. Our results revealed that uremic patients with HD had significantly higher blood levels of Ca, Mg, Zn and Pb and lower Cu, Fe, Se and Mn concentrations than healthy controls. In conclusion, a reproducible and reliable ICP-MS method using minimal whole blood sample volume (50 μL) with a simple dilution-based preparation procedure was successfully improved, validated and applied. Uremic patients undergoing long-term HD might be at increased risk of some essential trace elements deficiency (especially for Cu, Fe and Se) or toxic trace element excess (Pb) in respect to healthy subjects. Monitoring of blood levels and supplementation of some trace elements may be indicated in uremic patients undergoing long-term HD.
很少有研究报道长期（>24 个月）血液透析（HD）的尿毒症患者微量元素的变化，特别是使用全血作为测量目的的生物液体。我们的目的是开发一种改进的微量采样电感耦合等离子体质谱（ICP-MS）方法，以测定长期接受 HD 的尿毒症患者的全血中 Ca、Mg、Cu、Zn、Fe、Mn、Se 和 Pb 水平。建立了一种 ICP-MS 方法，对全血样本制备过程进行了改进，采用小体积，用于同时定量测定长期接受 HD 的尿毒症患者的各种元素。招募了 124 名符合条件的长期接受 HD 的尿毒症患者（75 名男性和 49 名女性）和 77 名健康受试者（54 名男性和 23 名女性），并进一步测定了 Ca、Mg、Cu、Zn、Fe、Mn、Se 和 Pb 水平。我们的结果表明，与健康对照组相比，接受 HD 的尿毒症患者的血液 Ca、Mg、Zn 和 Pb 水平显著升高，而 Cu、Fe、Se 和 Mn 浓度降低。总之，成功改进、验证和应用了一种使用最小全血样本量（50μL）和简单基于稀释的制备程序的可重复且可靠的 ICP-MS 方法。与健康受试者相比，长期接受 HD 的尿毒症患者可能存在某些必需微量元素缺乏（特别是 Cu、Fe 和 Se）或有毒微量元素过量（Pb）的风险增加。监测血液水平并补充一些微量元素可能对长期接受 HD 的尿毒症患者有益。
